慢性乙型肝炎（慢乙肝）严重危害人民健康。中医药在乙肝治疗中发挥着重要作用。中医证候客观化、标准化研究是对中医药临床疗效进行客观、科学评价的前提和基础。我们在依托于国家十二五科技重大专项（2012ZX10005001-004）研究中借助微阵列芯片和实时定量PCR方法进行检测，发现在慢乙肝肝胆湿热证和肝肾阴虚证患者血清中microRNAs存在差异表达；与肝肾阴虚证患者和健康者比较，在肝胆湿热证患者血清中miR-583和miR-663呈高表达(P<0.001)；ROC曲线分析显示，miR-583和miR-663能将肝胆湿热证和肝肾阴虚证明显区分开。本项目在前期研究基础上，运用分子/细胞生物学及生物信息学等技术手段开展miR-583和miR-663功能研究，揭示miR-583和miR-663分子调控机制。其研究对于阐释慢乙肝证候的形成机制、揭示不同中医治法作用原理等，具有重要的学术意义和应用前景。

Hepatitis B virus (HBV) infection is one of the major health problems in China. Traditional Chinese medicine (TCM) ZHENG as the key pathological principle is to understand the human homeostasis and guide TCM treatment. Our previous study was supported by National Science and Technology Major Project of China, No. 2012ZX10005001-004. We found that serum miRNAs were differentially expressed between Liver-gallbladder dampness-heat syndrome (LGDHS) and Liver-kidney yin deficiency syndrome (LKYDS). Circulating miR-583 and miR-663 were significantly higher (P<0.001) in CHB patients with LGDHS than those with LKYDS and healthy controls (Ctrls). ROC curve analysis revealed that miR-583 and miR-663 were sensitive and specific enough to distinguish LGDHS from LKYDS. MiR-583 and miR-663 may be potential markers for ZHENG differentiation in CHB. Further study will investigate the function of miR-583 and miR-663. It might help to elucidate the biological role in the progression of chronic liver diseases with TCM ZHENG.