心理应激可引起生殖内分泌系统紊乱、导致男性不育。课题组前期发现心理应激可导致雄鼠生殖损伤并引起β-内啡肽(β-EP)的释放，而纳洛酮（β-EP受体的拮抗剂）干预可逆转应激鼠的生殖损伤，提示心理应激可诱导β-EP导致雄鼠生殖损伤。本研究拟通过建立空白对照组、心理应激组、β-EP干预组、纳洛酮干预应激组、β-EP基因敲除应激组及对照组大鼠模型，并对其行为、生殖器官形态、精子质量、生殖细胞凋亡、生殖内分泌激素等多水平检测分析，明确β-EP导致应激鼠生殖损伤；采用lable free蛋白组技术分析各组雄鼠睾丸间质细胞的蛋白质表达全谱，通过组间多重比对分析、筛选差异表达蛋白，生物信息学方法分析其功能、通路及相互作用，采用免疫组化、Western blot及qRT-PCR技术多重验证筛选的关键蛋白，综合分析结果，阐明β-EP导致应激鼠生殖损伤的分子网络机制，为心理应激引起的男性不育理论机制提供新内容。

Psychological stress can induce disorders in male reproductive and endocrine system, and could lead to male infertility. Our previous study found that psychological stress induced reproductive damage of male rats, and released β-endorphin(β-EP), while stressed rat injected with naloxone (the antagonists of β-EP receptor) could reverse this damage. which suggested that psychological stress induced beta-EP and caused male reproductive damage. In the current study, we first aimed to verify the reproductive damage of male rat induced by psychological stress, by constructing a series rat models, which including the control group, the psychological stress group, β-EP intervention group, naloxone intervention stressed group, the stressed β-EP knockout and β-EP group, and also by analysis of the behavior, reproductive organs morphology, sperm quality, germ cell apoptosis, reproductive endocrine hormone level. And then to confirm that the reproductive damage of stressed male rat could induced by β-EP. The molecular network mechanism of male reproductive damage was based on proteomic study. The proteomic profiling of the leydig cells of all rat group were screened by label free technical, and comparison analysis of proteomic profiling between any two groups, the different expressed proteins were selected, their function, pathways and interaction were analyzed by bioinformatics methods, and the verification of their expression level could be performed by immunohistochemistry, Western blot and qRT- PCR analysis. Based on all above analysis, the molecular network mechanism of male reproductive damage caused by β-endorphin, which induced by psychological stress should be elucidated, and this research could provide a new theory for male infertility caused by psychological stress.