肿瘤特异代谢（TSM）是指包含瓦伯格效应（超高水平的有氧糖酵解）在内、悬殊于正常的代谢方式，它能为肿瘤恶性增殖和侵袭转移营造内环境和平衡原料能源供给，是其掠夺营养、耗竭器官必经的夺命途径，故成为晚期癌症延长带瘤生存期的重要切入点。最近2篇《Nature》论文报道，TSM还与转移的启动密切相关。中药复方调制复杂的TSM网络具有优势潜力，前期发现左金丸的主要成分吴茱萸碱在代谢层面有5个TSM抑制靶点，对已知TSM信号通路PI3K\Akt、HIF等有显著调制作用，吴茱萸碱小檗碱除广谱诱导凋亡外还能抑制内皮间质化转变（EMT）及调节相关蛋白表达，抑癌细胞迁移侵袭。本课题拟进一步研究左金丸及其主要成分体内外遏制肝癌TSM与机制及物质基础，研究其调制TSM与抑EMT、增殖转移之间的关系，是否因果而非伴随效应，以此为例据揭示调制TSM网络减低夺命性是中医药肿瘤临床取得延长带瘤生存期疗效的另一重要机制策略

Tumor-specific metabolism(TSM) including Warburg effect is a promising start point of targeted therapy. The crucial links in the process of intervention are in the extraordinary tumor glucose uptake, the key enzymes of glycolysis, pentose phosphate pathway, nucleotide synthesis and their regulatory pathways. Preliminary study found evodiamine significantly lowered the transcription level of glycolytic key enzymes , enzymes in the pentose phosphate pathway and catabolism of fatty acid, nucleoside diphosphate reductase and proteins in HIF and PI3K/AKT signal pathways. Evodiamine and berberine could also Inhibit tumor cells metastasis by Inhibitting EMT . Zuojinwan or its component, Evo (evodiamine) combined with cdk1 inhibitions;Coptis chinensis(berberine), showed synergistic anti-tumor effects either in vitro or in vivo,while sequential therapy . This study plans to use methods like quantitative real-time PCR, metabolite quantitative assay, Western Blot, enzyme activity assay , cloning over expressing and RNAi/special inhibition to clarify Zuojinwan or its component evodiamine and berberine and so on intervent TSM through inhibitting the enzymes and regulatory correlated pathways, to explicate its mechanism and relationship with tumor metastasis and extending lifetime with tumor-remained in bodies. In addition, to set an example for Chinese Medicine therapy of multi-target intervention of TSM.