Ox-LDL通过LOX-1浸润内皮细胞所致内皮功能障碍和内皮细胞生存危害对动脉粥样硬化的发展至关重要，但现仍无良好的特异性干预手段阻滞这一过程。我们的初步研究发现针对Ox-apoB特异性内皮粘附抗原表位的抗体可改善Ox-LDL通过LOX-1对内皮屏障所致的损害并减少内皮细胞凋亡；且主动免疫小鼠此片段可减轻粥样硬化的发生。据此我们推测以Ox-apoB特异性片段行免疫干预可阻滞Ox-apoB通过LOX-1引起的内皮损伤和脂质沉积，抑制粥样硬化及相关损害。据此，本研究拟采用主动及被动免疫策略观察动物内皮细胞屏障连接、焦亡及凋亡等生存状态及斑块形成的保护作用，并以LOX-1敲除鼠验证LOX-1介导此致病通路；同时，对上述效应及作用机制在离体内皮细胞上进行系列验证；本研究还试图借此探讨此免疫策略对支架内新生动脉粥样硬化有无作用。最终从内皮细胞保护的角度为动脉粥样硬化及相关损害提供可行的免疫干预途径。

The endothelial dysfunction and endothelial cell survival state mediated by Ox-LDL infiltrating endothelial cells through lectin-like oxidized low desity lipoprotein receptor-1 (LOX-1) play a critical role in the pathogenesis of atherosclerosis and related lesions. However, there are still no favorable and-specific interventive methods to block this pathological process. Our preliminary study found that the antibodies against Ox-apoB specific peptide fragments related endothelial epitopes could improve Ox-LDL related endothelial cell junction barrier damage and reduce endothelial cell apoptosis through LOX-1 pathway, and actively immunize mice with this Ox-apoB specific peptide fragments can reduce the development of atherosclerosis. On these grounds, we hypothesized that the immunization treatment with Ox-apoB specific fragments could block Ox-apoB related endothelial damage and lipid deposition through LOX-1, and inhibit the development of atherosclerosis and related lesions. Therefore, this study is intended to protect endothelial cell barrier junction, prevent endothelial cell pyroptosis and apoptosis, improve endothelial cell survival state by active and passive immunization strategies with this Ox-apoB specific fragments and observe protective effect on the atherosclerosis plaque formation and related lesions. And we will use LOX-1 knockout mice to verify this process involved LOX-1 pathway; at the same time, the above effects and mechanisms will be verified in endothelial cells in vitro. And this study also tries to investigate the effects of immunization strategiest on the pathogenesis of neoatherosclerosis. Finally, the main goal of this study is to explore the feasible immune intervention way to prevent the pathogenesis of atherosclerosis and related lesions through protection mechanisms of the endothelial cells.