胃癌腹膜转移是肿瘤复发及术后死亡主要原因之一。研究表明腹膜转移发生发展与Akt/NF-κB通路介导上皮间质化改变有关，但该通路分子调控及如何调控下游信号尚不明确。我们前期研究显示CIP2A与胃癌腹膜转移相关并可引起Akt/NF-κB相关信号分子表达改变，同时发现影像技术联合运用如双能CT及PET-CT可侦测sFRP1基因表达改变促进胃癌腹膜转移形成，且同步辐射相衬成像能检测微米级肿瘤新生血管，提示多模态影像学对肿瘤组织微结构和基因分子水平评估具很大潜力。本课题组将利用多模态影像学和分子生物技术相结合：1）探索CIP2A介导Akt信号通路引起腹膜转移发生发展分子机制；2）提高多模态影像技术对腹膜转移早期检出率及肿瘤上皮间质化和血管生成准确评估率；3）通过多模态影像技术验证CIP2A对腹膜转移靶向治疗分子干预效应。该研究结果将为探究胃癌腹膜转移分子机制、早期检出和靶向治疗疗效评估提供新思路。

Gastric cancer peritoneal metastasis is one of the main reasons for the tumor recurrence and postoperative death.Akt/NF-κB signaling pathway regulating the change of Epithelial-mesenchymal transition plays a role in the development of gastric cancer peritoneal metastasis,while the molecular mechanism of how Akt/NF-κB influences the molecular regulation and targeted therapy of gastric cancer peritoneal metastasis remains unclear.Our initial findings implied that CIP2A could regulate the occurrence of the process and lead to a change of Akt/NF-κB related signaling molecules,and the combination of imaging techniques like Dual-energy CT and PET-CT can detect the gastric cancer peritoneal metastasis formation through the expression of sFRP1 gene,and also found the synchrotron radiation phase contrast imaging can detect micron grade tumor angiogenesis.Using multimodal imaging methods have great potential to evaluate the tissue microstructure and genetic molecular information of the gastric tumor.We will combine multimodal imaging methods and the molecular biological techniques, 1) to explore the molecular mechanisms in the activation of the Akt signaling pathways associated peritoneal metastasis ; 2) to improve the evaluation of early peritoneal metastasis detection and tumor molecular bio-information by the multimodal images; 3) to evaluate the outcomes of gene regulated the peritoneal metastasis targeting therapy by multimodal imaging approach.Our results will provide a novel approach to explore the molecular mechanisms,early detection and targeted therapy evaluation of peritoneal metastasis.