Identification of microRNAs Actively Involved in Fatty Acid Biosynthesis in Developing Brassica napus Seeds Using High-Throughput Sequencing.
Identification of microRNAs Actively Involved in Fatty Acid Biosynthesis in Developing Brassica napus Seeds Using High-Throughput Sequencing.
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利用高通量测序鉴定在开发甘蓝型油菜种子时积极参与脂肪酸生物合成的 microRNA
DOI：
10.3389/fpls.2016.01570
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发表时间：
2016
影响因子：
5.6
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Wang J;Jian H;Wang T;Wei L;Li J;Li C;Liu L
种子发育在种子植物的生活史中起着至关重要的作用。油菜是一种重要的油料作物，脂肪酸在胚胎发育过程中合成并储存于特定的组织中，了解种子发育过程中脂肪酸合成的分子机制是一个重要的研究目标。在这项研究中，我们构建了三个小RNA文库，从早期种子在开花后14，21和28天，并使用高通量测序来检查microRNA（miRNA）的表达。共鉴定出30个家族的85个已知miRNAs和1160个新miRNAs，其中24个miRNAs参与脂肪酸的生物合成，包括5个已知miRNAs和19个新miRNAs，分别为bna-miR 156 b、bna-miR 156 c、bna-miR 156 g、novel_mir_1706、novel_mir_1407、novel_mir_173、和novel_mir_104在21 ℃和28 ℃显著下调，而bna-miR 159、novel_mir_1081、novel_mir_19和novel_mir_555显著上调。此外，我们发现一些miRNA调节直接参与脂肪酸生物合成的功能基因，而另一些miRNA通过作用于大量转录因子来调节脂肪酸生物合成过程。通过定量RT-PCR对miRNAs及其相应的预测靶点进行了部分验证。我们的数据表明，不同的和复杂的miRNAs参与种子发育过程，miRNAs在种子发育过程中的脂肪酸生物合成中发挥重要作用。
Seed development has a critical role during the spermatophyte life cycle. In Brassica napus, a major oil crop, fatty acids are synthesized and stored in specific tissues during embryogenesis, and understanding the molecular mechanism underlying fatty acid biosynthesis during seed development is an important research goal. In this study, we constructed three small RNA libraries from early seeds at 14, 21, and 28 days after flowering (DAF) and used high-throughput sequencing to examine microRNA (miRNA) expression. A total of 85 known miRNAs from 30 families and 1160 novel miRNAs were identified, of which 24, including 5 known and 19 novel miRNAs, were found to be involved in fatty acid biosynthesis.bna-miR156b, bna-miR156c, bna-miR156g, novel_mir_1706, novel_mir_1407, novel_mir_173, and novel_mir_104 were significantly down-regulated at 21 DAF and 28 DAF, whereas bna-miR159, novel_mir_1081, novel_mir_19 and novel_mir_555 were significantly up-regulated. In addition, we found that some miRNAs regulate functional genes that are directly involved in fatty acid biosynthesis and that other miRNAs regulate the process of fatty acid biosynthesis by acting on a large number of transcription factors. The miRNAs and their corresponding predicted targets were partially validated by quantitative RT-PCR. Our data suggest that diverse and complex miRNAs are involved in the seed development process and that miRNAs play important roles in fatty acid biosynthesis during seed development.
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玉米种子萌发过程杂种优势的 MicroRNA 转录组分析
影响因子：
3.7
作者：
Ding D;Wang Y;Han M;Fu Z;Li W;Liu Z;Hu Y;Tang J
通讯作者：
Tang J
影响因子：
4.4
作者：
Han R;Jian C;Lv J;Yan Y;Chi Q;Li Z;Wang Q;Zhang J;Liu X;Zhao H
通讯作者：
Zhao H
影响因子：
5.1
作者：
Golz, John F.
通讯作者：
Golz, John F.
miRNA 和 siRNA 在植物生物和非生物胁迫反应中的作用。
DOI：
10.1016/j.bbagrm.2011.05.001
发表时间：
2012-02
影响因子：
4.7
作者：
Khraiwesh, Basel;Zhu, Jian-Kang;Zhu, Jianhua
通讯作者：
Zhu, Jianhua
影响因子：
7.4
作者：
Focks, N;Benning, C
通讯作者：
Benning, C