血管支架置入术是狭窄型动脉粥样硬化常见且有效的治疗手段，但是其术后血栓的发生机理至今不明。尽管如此，前人研究发现一氧化氮在血栓的形成中扮演着重要的角色。由此，申请人认为血管支架植入后力学环境变化会改变血管内一氧化氮的分布，进而影响支架内血栓的形成。为了验证该推测，本项目拟开展以下研究：（1）通过体外仿真实验技术获得支架植入血管后一氧化氮的释放规律；（2）构建在体和体外血管-血流-支架间相互作用的力学模型，探讨支架植入血管后力学环境（流动剪切应力、血管壁内应力分布等）的变化规律，并基于（1）中结果分析力学环境的改变对一氧化氮分布规律的影响；（3）研究支架植入后一氧化氮分布的改变对宿主血管内血小板粘附、活化的影响。本研究不仅能为支架内血栓形成机理提供新的研究思路，也为新型血管支架的设计提供理论依据。

Endovascular stent implantation is an effective and widely utilized therapy for atherosclerotic stenosis, but the mechanisms of in-stent thrombus are still not clearly known. Nevertheless, it has been documented that nitric oxide (NO) play an important role in the formation of thrombus. The applicant hypothesizes that the stent-induced alteration in the mechanical environment of the host artery can affect NO concentration and disturb its distribution, hence contributing to in-stent thrombus. To verify the hypothesis, this proposed research project will carry out the following studies: (1) Develop an experimental hemodynamic system in vitro to measure the NO production rate in the stented artery. (2) Develop the artery-blood-stent interaction mechanical model after stenting; and simulate in vivo and vitro the regularity of the stent-induced alteration in local vascular mechanical environment (flow shear stress, the stress distribution in the blood vessel wall etc); based on (1), study on the distribution of NO due to the alteration of mechanical environment in the stented artery. (3) Based on the study of (2), verify if the distribution of NO in host artery would affect the platelet adhesion and activation. This study might not only provide new ideas to clarify the mechanism of in-stent thrombus, but also provide a theoretical basis for the design of new stents.