具有疏肝化痰熄风功效的加味柴胡疏肝汤治疗癫痫有效，但机制不清。申请者在前期研究中发现，癫痫小鼠海马组织中miR-187下调，其靶基因MAPK8与Wnt凋亡通路密切相关；而癫痫小鼠海马组织中高表达的lncRNA-UCA1能够通过结合miR-187，激活Wnt通路，从而促进海马神经元细胞凋亡，发生癫痫。因此我们假说该方可能通过抑制UCA1/miR-187/MAPK8/Wnt通路而抑制海马细胞凋亡，控制癫痫。本研究拟构建癫痫小鼠模型，应用qRT-PCR、Western blot、免疫组化等技术观察UCA1/miR-187/MAPK8/Wnt通路上相关基因UCA1、miR-187、MAPK8、β-catenin、cyclin D1、c-myc、wnt3、Bax、Bcl-2的表达变化及加味柴胡疏肝汤干预后对上述基因表达的影响，探讨该方治疗癫痫的内在机制，夯实临床基础，为癫痫的防治提供新靶点和新方法。

Modified Chaihu Shugan Decoction With the effect of the function of soothing liver, resolving phlegm and extinguishing wind is effective in treating epilepsy, but the mechanism is not clear. Previous researches of our group have founded that miR-187 is down-regulated in hippocampus of epileptic mice, and its target gene MAPK8 is closely related to Wnt/β-catenin apoptosis pathway; And the high expression of LncRNA-UCA1 in the hippocampus of epileptic mice can activate the Wnt signal pathway by binding to miR-187, and thereby promote apoptosis of hippocampal neurons and have seizures. We hypothesize that the prescription may inhibit UCA1/miR-187/MAPK8/Wnt signal pathway and inhibit hippocampal cell apoptosis and control epileptic seizures in consequence. The purpose of this study is to establish the mouse models of epilepsy, to observe expression changes of UCA1, miR-187, MAPK8, β-catenin, cyclin D1 , c-myc, wnt3, Bax, Bcl-2 and so on which related with UCA1/miR-187/MAPK8/Wnt signal pathway, and the effects of the expression of the above genes after the intervention using Modified Chaihu Shugan Decoction on with qRT-PCR, Western blot, immunohistochemistry and other techniques. To explore the internal mechanism of treating epilepsy with Modified Chaihu Shugan Decoction and lay a solid foundation for its better clinication, at the same time, it also provides a new target and method for the prevention and treatment of epilepsy.