深度烧烫伤常导致创面皮肤剧痛、坏死、感染、瘢痕等难症，目前缺乏高效治疗药物，且相关药物的治疗机制尚未明了，反过来阻碍高效新药的开发。为此，本项目针对Ⅲ度烫伤皮肤具有明显治疗功效的中药复方生肤油，研究其促进深度烫伤皮肤无瘢痕修复的分子机制。分别建立Ⅲ度烫伤动物模型、毛囊干细胞和成纤维细胞的不同培养系以及抗感染检测体系，利用免疫组化、核酸定量等技术，检测烧烫伤后创面及周边皮肤组织中以及各培养体系中β连环素、P物质等蛋白、核酸的表达量，研究其对毛囊干细胞增殖与分化、成纤维细胞凋亡抑制等过程的调控关系，揭示β连环素、P物质共同调控创面早期再生及后期瘢痕修复的基本规律，并研究生肤油影响β连环素与P物质间信号传导并促进皮肤"生肌、祛瘀"的规律，阐明生肤油促进Ⅲ度烫伤创面皮肤无瘢痕修复的分子机制。本项目的研究结果有助于更好地从理论上认识创伤皮肤的修复过程，为烧烫伤治疗新药的开发提供新的靶点。

Deep thermal injuries result in the loss of a physical barrier to pain, infection, progressive necrosis and scar formation. However, there is no a better understanding of the molecular mechanisms behind these processes may provide insight into developing improved wound healing therapies. ShengFu Oil is a Chinese herbal compound that is widely used to treat various burns and scalds. Our previous studies suggest that SFO could stimulate the skin's regeneration and help to reduce excess scars and to enhance scarless repair. It is therefore urgent to research the mechanism of the skin regeneration and scarless repair by SFO. In this project, we will determine the expressions of β-catenin and substance P in the skin tissues of full-thickness burn model during wound repair using some techniques including radioimmounassay, image analysis and RT-PCR, etc. Furthermore, we will investigate the mRNA and protein expression levels of those factors described above and analyze the relationship between β-catenin and substance P, and investigate influences of those factors on the cell proliferation of rat cultured hair follicle stem cells and the apoptosis inhibition of fibroblasts derived from human pathological scars. The results will conclude the co-regulatory effects of β-catenin and substance P on the skin regeneration and wound scar healing. Next, we will investigate the regulatory effects of SFO on the signal transduction between β-catenin and substance P, and to identify SFO able to accelerate "Shengji" and "Quyu" in the skin tissues of full-thickness burn wounds. The results described above will clarify molecular mechanism of scarless wound healing enhanced by SFO. The results of this project contributes to a better understanding of skin wound healing theory, provide new targets for the development of drugs for the treatment of burn wounds.