对乙酰氨基酚(Acetaminophen)是广泛应用于临床的解热镇痛非处方药，其过量使用是导致美国和欧洲由药物引起急性肝功能衰竭的首要原因。我国含有AP的非处方药物多达数百种，易发生患者过量服用的情况，但我们对AP肝毒性还没有充分的认识。配伍是中医用药的特色之一，也是中医理论的精髓之一，通过配伍不仅可以协同促进药效，还能减少药物毒副作用。我们前期研究发现常与AP联合应用的中药金银花及其主要成分绿原酸可以抑制AP的肝毒性，进一步研究发现细胞内GSH和Trx抗氧化系统以及丝裂原活化蛋白激酶信号通路共同参与调控绿原酸抑制AP肝毒性的作用。本项目主要在前期工作基础上，通过双重调控转录因子Nrf2和Egr1信号通路揭示绿原酸抑制AP肝毒性的机理；研究其对AP肝毒性的治疗解毒作用；探索其对AP解热镇痛药效活性的影响，为AP肝毒性的防治提供新的解毒策略，也为含绿原酸中药和AP的联合应用提供实验证据。

Acetaminophen (AP) is a widely used over-the-counter (OTC) analgesic and antipyretic drug. Hepatotoxicity due to AP overdose is the most common cause of acute liver failure in both the United States and the United Kingdom. Numerous drugs and Chinese patent medicines in the market contain AP, and most of them are indexed in the OTC directory in China. In some cases, patients use a variety of such drugs, which will increase the possibility of AP overdose. However, at present AP-induced hepatotoxicity has not been fully recognized in our country. Our previous study has found that Flos Lonicerae, which is commonly combined with AP used for cold and fever, and its main ingredient chlorogenic acid, can prevent AP-induced liver injury. Further results show that cellular GSH and Trx antioxidant system, and MAPK signaling cascades participate in the prevention of chlorogenic acid against AP-induced liver injury. Based on the previous research, the present study aims to observe whether chlorogenic acid prevents AP-induced liver injury by dual regulating transcription factor Nrf2 and Egr1 signaling pathway, to further observe the therapeutic detoxification of chlorogenic acid against AP-induced liver injury, to observe the effect of chlorogenic acid on the analgesic and antipyretic activity induced by AP. This study will provide new strategy for the detoxification of AP-induced liver injury, and also provide the experimental basis for the combined application of AP and traditional Chinese medcicines, which contain chlorogenic acid.