既往报道的各类组织工程血管（TEBVs）均存在构建程序复杂、等待时间较长等问题，无法适用于临床急诊和限期手术，因此构建一种兼顾安全性与功能性的off-the-shelf(即用型) TEBVs具有较大临床实用价值。申请人结合自身工作基础，拟采用同种异体细胞自组装、脱细胞及肝素固化等技术，可控性制备具有良好物理性能与血液相容性的人源细胞外基质作为血管支架；以直接提取的成人脂肪干细胞（ADSCs）作为种子细胞；并应用电场诱导技术使ADSCs于支架腔内趋向性黏附生长、提高细胞种植效率，构建全生物化off-the-shelf TEBVs，预计总体构建时间<2h。通过裸大鼠腹主动脉移植实验观察人源TEBVs移植后通畅率、内膜增生与结构重塑等组织形态学特征，并从DNA、RNA和蛋白质水平检测、评价种子细体内保持与分化，以及工程化血管的生理功能等。本课题的顺利实施可促进实验TEBVs向临床应用进一步转化。

Current various types of tissue engineering blood vessels (TEBVs) possess complicated construction procedure and long waiting time, and can not be applied to emergency or time-limited surgery (eg, emergency coronary artery bypass grafting, critical lower limbs ischemia, etc.), therefore, to construct a safe and functional off-the-shelf TEBVs will have great clinical value. Based on our previous work, we propose to combine allogeneic cells self-assembly, decellularization and heparin immobilization techniques to fabricate a controllable human extracellular matrix as a scaffold material with good physical property and blood compatibility. The adult adipose stem cells (ADSCs) extracted directly but without culture in vitro will be used as seeding cells. We will apply electric field induced technique to enable ADSCs to migrate and adhere on the luminal side of the scaffold, in order to improve the efficiency of cell seeding, and construct completely biological off-the-shelf TEBVs, expecting that overall constructing time will be less than 2h. There after, the human TEBVs (hTEBVs) will be transplanted into a nude rat abdominal aortic aorta, and the tissue morphology characteristics, such as patency rate, intimal hyperplasia and remodellation after transplantation, will be observed. Through the detection from DNA, RNA and protein level, the keeping and differentiation of the seeding cells and the physiological function of engineered blood vessels will be evaluated. The successful implementation of this subject might promote the further conversion of experimental TEBVs to the clinical application.