鉴定CLV信号通路新成员

茎端分生组织的稳态平衡对植物进行正常的形态建成至关重要。因此植物在进化过程中建立了精细的调控机制来保证茎端分生组织的稳态，其中CLV-WUS这条负反馈调节回路起着决定性的作用。但由于茎端分生组织的结构非常精细，细胞群体很小，很难进行深入的机理研究。我们前期的工作利用cik1 2 3 4 5 6六重突变体极端膨大的茎端分生组织，构建了特异的茎端分生组织酵母文库，期望通过筛选CIKs和MILs的相互作用蛋白来鉴定到CLV信号通路的新成员。另外，由于基因的功能冗余，传统的通过loss-of-function进行遗传筛选，很难再鉴定到新的调控元件，于是我们构建了过表达的突变体库，期望通过gain-of-function筛选到新的突变体。目前我们已经从中筛选到了一个突变体rec1 (resemble clavata 1)，茎端分生组织膨大，并且出现显著的多心皮角果，后续我们将解析背后的调控机理。

The homeostasis of shoot apical meristem (SAM) is crucial for proper morphogenesis. Plants have evolved elaborate genetic networks to ensure the balance between cell division and differentiation, thus to control meristem size and stem cell maintenance. Among them, the CLV-WUS negative feedback loop plays an essential role. But due to the fine structure and small cell population of the SAM, it is hard to get enough materials for further research. Our previous work have generated the cik1 2 3 4 5 6 sextuple mutant, which display extremely enlarged SAM that is larger than all clv mutants. Take advantage of the huge meristem of cik1 2 3 4 5 6，we construct a specific yeast library of SAM. Through screen the interaction protein of CIKs and MILs, we may identify new components of CLV pathway. In addtion, due to the gene redundancy, genetic screen for loss-of-function is hardly to identify new components.We construct an overexpression library for screen the gain-of-function mutants. We have obtained a mutant that show enlarged SAM and carry more than two carpels per flower, so it is named rec1 (resemble clavata 1). In the future, we will elucidate the mechanism of REC1 in regulating the homeostasis of SAM.