大肠杆菌是一种生活在人体肠道内的细菌，与人类息息相关，它被作为一种模式生物用来研究单细胞的运动行为。对大肠杆菌运动行为的研究不仅具有基础科学上的意义，同时也有广阔的应用前景。葡萄糖作为大肠杆菌重要的能量来源，其在大肠杆菌生命运动过程中具有重要的意义。本项目拟采用生物物理学技术和手段，从新的角度研究大肠杆菌K-12对葡萄糖的运动动力学响应。借助细菌游动3D-tracking技术、乳胶小球标记细菌鞭毛技术以及微流控技术等，研究大肠杆菌K-12在不同葡萄糖环境下的运动动力学过程；通过分析大肠杆菌鞭毛马达的动力学特征（转动速度、力矩），大肠杆菌的趋化运动性（游动速度、翻转频率、游动距离），系统研究大肠杆菌对葡萄糖的运动动力学响应。并进一步通过基于趋化网络和鞭毛马达的生物物理模型对其运动动力学响应进行机制分析。该研究不仅加深对大肠杆菌运动动力学过程的理解，同时也丰富肠道微生物对葡萄糖响应的理论依据。

Escherichia.coli (E.coli) is a bacterium that lives in human’s gut and is closely related to human beings. It was also served as a model organism to study the motion behavior of single cell. The research on E.coli motion behavior not only exists the fundamental significance, but also shows the widely application prospect. Glucose, which acts as the important energy source to E.coli, plays a crucial role in the motion of E.coli. This project was designed to take biophysical techniques and methods to study the effect of glucose on motion dynamics of E.coli K-12 from a new point of view. In this project, we are planning to research the motion dynamics of E.coli K-12 under various glucose concentration solution environments by using bacteria 3D-tracking, latex bead labeling to the filament of bacteria, as well as micro-fluidics and so on; to systematically reveal the motion dynamics response of E.coli to glucose through analyzing the switching dynamics (rotating speed, torque) of E.coli flagellar motor, and the motility of E.coli (swimming speed, tumbling frequency, swimming distance) along with the chemotaxis. To further the mechanism study on the motion dynamics of E.coli by combining biophysical models based on chemotaxis network and flagellar motor. This project will not only reinforce the understanding of the motion behavior of E.coli, but also enrich the theoretical fundamentals of enteric microbiota response to glucose.