鉴于前期大量活性筛选发现甘遂和圆根大戟中的查尔酮类成分具有显著地保护多巴胺所致的神经细胞损伤作用，并并已发现其中查尔酮类成分为其活性成分。在此基础之上，本课题拟对其活性最好的化合物gansuione B (2)保护多巴胺所致的SH-SY5Y细胞损伤的分子机制进行研究，探明其保护多巴胺损伤的基因、蛋白及其相互之间的激活和调控关系。并利用6-羟基多巴（6-OHDA）诱发的大鼠PD模型，探讨gansuione B (2)保护PD大鼠的分子作用靶点。通过对查尔酮类成分进一步的分离纯化，并利用合成和半合成手段制备系列衍生物，根据分子作用靶点对系列查尔酮类化合物进行构-效关系研究，以期为新型PD治疗药物的发现提供新药先导化合物和新的作用靶点。而进而利用UPLC-TOF-MS建立查尔酮类化合物含量测定方法，筛选新的药用资源植物，并通过对圆根大戟和甘遂栽培研究，为新药研究开发提供药用资源。

In our previous research, it was found that the chalcones in Euphorbia kansui and Euphorbia pekinensis Rupr. prevented the dopamine damage to nerve cells. On these foundations, we except to prove up how gansuione B (2) with the strongest biological acivity prevented the dopamine damage to SH-SY5Y nerve cells, and investigate the relations between the genes and the proteins prevented the dopamine damage such as activation and regulation. The PD rats induced by 6-OHDA will be used to explore how gansuione B (2) protect the molecule target of PD rats. Afterwards the chalcones were isolated and purified, the derivatives might be prepared by synthetic and semi-synthetic methods. The structure-activity study of chalcones will be based on molecule targets. And then the content determination method of the chalcones would be established by UPLC-TOF-MS in order to survey new medicinal plants resources. Meanwhile, the cultivation research of Euphorbia kansui and Euphorbia pekinensis Rupr. might provide basic technical supports for research and development of new medicinal resources.