呼吸道病毒感染所致小儿肺炎有逐年增高趋势。最常见呼吸道病毒IV、RSV诱导的肺部炎性损伤的病理基础，主要是肺微血管内皮细胞(PMVECs)通透性升高所致肺损伤。前期临床发现清肺通络敷胸膏能促进肺部啰音吸收，实验研究表明改善肺微循环。因而提出假说"基于肺热络瘀理论的清肺通络法干预病毒性肺部炎性损伤与PMVECs通透性调控存在一定相关性"。为此本研究以敷胸膏有效物质群为切入点，采用单克隆抗体技术定量分析。结合UPLC/MS-MS以及微透析技术进行整体和局部药动学研究，探寻物质基础及作用靶器官。通过析因设计，以肺部炎性损伤小鼠模型为对象，采用Western-blot等技术方法，观察MAPKs信号转导通路的动态变化，阐明呼吸道病毒所致PMVECs通透性升高的具体分子机制，探讨敷胸膏（主、辅药）药能效果差异，揭示"药辅共生"整合调节机制，为外治中药在病毒性肺部炎性损伤临床应用提供实验依据与基础。

Respiratory viral infections due to pneumonia in children are increasing year by year. The pathology characteristics that pulmonary inflammatory injury (PIFI) induced by the most common respiratory viruses IV, RSV are mainly owing to increased permeability of pulmonary microvascular endothelial cells (PMVECs). Early clinical research found that Fuxiong Paste had effect of clearing lung heat and meridians, promoting pneumonia rale absorption, and improving pulmonary microcirculation. Therefore,we proposed hypothesis that there is a certain correlation of traditional Chinese medicine external treatment with skin-meridians pathway and PMVECs permeability regulation. The purpose of this study will apply monoclonal technology in researching effective substance groups of Fuxiong Paste as the breakthrough point,carry the research on pharmacokinetics by UPLC/MS-MS and microdialysis technique to explore the substance basis and targets of the external medicine. Furthermore, Estabilsh mouse model of PIFI, and observate the dynamic changes of transduction pathway (MAPKs) by the technology of Western-blot and so on. Moreover, reveal the specific molecular mechanism of PMVECs permeability increase induced by respiratory viral, and from the disease-network, investigate Fuxiong Paste (main, auxiliary medicine) potency differences, developing the integrated mechanism of "medicine and auxiliary symbiosis" and provide the scientific experimental basis and foundation for the external treatment of Chinese medicine in the clinical application of viral lung inflammatory injury.