流感病毒（IV）和肺炎链球菌（SPn）共感染所致小儿肺炎在临床上常伴有肠道菌群失调，给治疗带来很大困难，究其关键机制与ROR相关信号通路肺肠共表达引起肺部炎性损伤及肠道微生态失衡密切相关。研究提出“毒损肺肠与共感染致肺肠免疫损伤相关”的假说。开肺调肠法以经典中药宣白承气汤为代表方剂临床疗效确切，其组分配伍清调兼施。本研究通过析因设计，以小鼠IV、SPn共感染模型为研究对象，采用蛋白组学差异蛋白质谱分析、双向电泳及Western-blot等技术方法，观察ROR相关信号转导通路及细胞因子群的动态变化，阐明流感病毒和肺炎链球菌共感染致肺部炎性损伤及肠道微生态失衡分子机制；同时以宣白承气汤活性成分为干预因素，结合 LC/ESI-MS/MS及UPLC/MS-MS技术，探寻其防治机制、药效物质基础及作用多靶器官，为中药在干预小儿混合感染肺炎及肠道菌群失调的临床应用提供依据。

Pneumonia coinfected by influenza virus (IV) and streptococcus pneumoniae (SPn) is commonly accompanied with alteration of intestinal flora in children, which is very difficult to locate the balance. Its major mechanism is that lung inflammatory injury and intestinal microecological imbalance, which is relevant to coexpression of retinoid acid receptor related orphan receptor (ROR) relevantsignal pathway in lung and large intestine. So we proposed the hypothesis that TM theory of collateral injury by pathogenic factors coincided with the mechanism of mucosa limmunologic injury in pneumonia coinfected by IV and SPn .Classic TM compound “Xuanbai Chengqi Tang (XBCQT)” had a good therapeutic effect, which was the representive formula of treatment guideline of dispersing lung and regulating large intestine. As its name, it can clear lung-heat and regulate large intestine at the same time.So a factorial design will be used to observe dynamic change of ROR relevant signal pathway and cytokines byprotein mass spectrometry analysis, two-dimensional electrophoresis and Western-blot analysis in mixed infected mice. We will illuminate molecular mechanism of lung inflammatory injury and intestinal microecological imbalance caused by coinfection of IV and SPn. Through LC/ESI-MS/MS and UPLC/MS-MS, we will explore the intervening mechanism, pharmacodyamic material basis and target of XBCQT to provide experimental basis for its clinical application.