迄今为止发现数量尚极其有限的古菌病毒群体却已展现了极为丰富的多样性，对它们的研究正使人们对病毒的起源、分类和对生命进化的影响形成新的认识。此外，目前报道的古菌病毒大多分离自环境，有关溶原病毒的研究尚很少。SNJ1和SNJ2是能共存于同一嗜盐古菌细胞的二个有包膜的古菌溶原病毒，其前病毒分别以质粒和整合在染色体上的方式存在。SNJ1能侵染染色体相同但不携带其前病毒质粒pHH205的菌株J7-2或CJ7，形成噬菌斑；而形态观察及基因组结构分析发现，SNJ2与分离自环境的一些古菌“烈性”病毒关系密切；在其它很多嗜盐古菌基因组上也预测到了SNJ2-like前病毒的普遍存在；前期实验还发现SNJ1和SNJ2之间可能存在相互作用。本申请拟对这二个新病毒的基本生物学特性、两病毒之间以及它们与宿主间的相互关系开展深入研究，进一步揭示古菌病毒的独特性质及生物学价值；並为发现更多的古菌溶原病毒奠定新的技术基础。

Although limited in isolate number, archaeal viruses have already represented abundant diversity. The study of archaeal viruses is providing reveal new insight into the origin and evolution of viruses, as well as viral classification. On the other hand, the documented haloarchaeoviruses are overwhelmingly isolated from environments, but rarely lysogenic viruses at present. We have isolated two membrane-containing lysogenic viruses SNJ1 and SNJ2 from haloarchaeon Natrinema sp. J7-1 by mitomycin C induction. The provirus of SNJ1 is the plasmid pHH205, and genome of SNJ2 integrate into host chromosome. SNJ1 can infect derivative strains J7-2 and CJ7 that free of plasmid pHH205, and produce plaques on their lawns. The morphology and the genome analysis revealed relatedness between SNJ2 and viruses isolated viruses from environment. And SNJ2-like proviruses are identified prevalent insertion in haloarchael chromosomes. MMC induction of strains containing different proviruses suggest there exit interactions between the two viruses. To expand our awareness for archaeal viruses, we intend to study on the biological properties of the two lysogenic viruses, and the relationships between viruses and their host cells. Our attempts will also set up methods for discovery and research on more archaeal lysogenic viruses.