本项目采用酶催化组装合成新型肽类纳米材料，以酶催化组装体系设计、过程调控与强化、机理剖析及材料应用为研究主线，旨在构建多种肽类分子酶催化组装新体系、新过程，阐明肽类分子酶催化组装机制，获得多种新型肽类纳米材料，并完成其应用。具体研究中：①体系设计和过程调控：设计多种新型肽类分子及酶催化组装新体系；从传质、酶催化和分子组装三方面，设计酶催化组装过程调控与强化策略，确定关键参数；②机理剖析和动力学：测试计算酶催化组装过程的热力学参数，剖析酶催化特性和分子组装机制；跟踪酶催化组装过程中产物含量和组装体结构的变化，构建动力学模型；③材料应用和性能强化：构建载药和传感分析（光学、电化学）应用体系，并设计材料性能强化策略，提升材料的应用潜力，建立多种药物缓控释系统和传感分析方法。本项目的研究方法和成果可为新型肽类分子酶催化组装设计、过程调控/强化以及肽类纳米材料应用开发提供有益的理论指导与实践参考。

In this proposal, simultaneous enzymatic hydrolysis and self-assembly (enzymatic-assembly) was proposed to prepare novel peptide-based nanomaterials. To establish novel enzymatic assembly systems, understand the reaction-assembly mechanisms, and obtain novel peptide-based functional materials, we plan to focus on the study of the design of enzymatic assembly systems, process control and enhancement, the analysis of the mechanism, and the applications of these materials. (1) Process design and control: we plan to design some novel peptide-based molecules and their corresponding enzymatic-assembly processes, as well as to control and improve the process based on the mass transfer, enzymatic reaction and supramolecular self-assembly. (2) Mechanism analysis and dynamics: we plan to test the thermodynamics data of the enzymatic-assembly process, and analysis the feature of enzyme action and the self-assembly. In addition, we also try to characterize the change in the peptide products and the structure of assemblies, as well as to establish a suitable dynamical model. (3) Applications and performance improvement: we plan to investigate the applications of these synthesized nanomaterials in drug delivery and sensors (optical and electrochemical sensors), as well as to improve the application performance, aiming of establishing some novel drug delivery systems and sensor analysis methods. The methodologies in this project and its prospective results can open an avenue for the design of new enzymatic-assembly systems for the nanomaterials synthesis, process control for enzymatic-assembly, and the applications of peptide-based materials.