颗粒物-蛋白质相互作用研究是环境与健康领域的前沿和难点之一。本项目以黏蛋白和淀粉样蛋白两类与重大疾病密切相关的蛋白质为研究对象，从分子、超分子尺度上研究纳米颗粒、大气颗粒物与蛋白质的相互作用。具体研究中：①考察颗粒物在黏蛋白表面的吸附-脱附行为及其对黏蛋白性能（润滑、抗污染）的影响，根据蛋白质结构、相互作用力大小等参数，结合理论计算模拟，剖析颗粒物-蛋白质相互作用机理；②组合光谱/波谱学、电镜学等，跟踪表征淀粉样蛋白的成核和纤维化过程，对比分析颗粒物对蛋白质聚集行为的影响，统计分析不同颗粒物特性对蛋白质聚集的影响规律，并剖析蛋白质聚集机理及颗粒物的影响机制；③开发颗粒物在黏蛋白表面的脱附过程强化、抑制蛋白质淀粉样聚集/诱导蛋白纤维解聚的新方法，消除颗粒物对蛋白的毒性影响。本项目的研究方法和成果可为深入理解颗粒物生物学效应及毒性机制提供有益的参考和丰富的实验依据。

The interaction between particles and proteins is central to many aspects of environment and health fields. In this proposal, two types of proteins, mucoprotein and amyloid proteins, which are closely related with many serious diseases, are chosen as the models. The interaction between nanoparticles/ atmospheric particulates and proteins will be comprehensively investigated at the molecular and supramolecular level. Specifically, 1) we plan to study the adsorption-desorption behavior of particles on the mucoprotein surfaces and the effect of the deposited particles on the lubrication/antifouling performance of proteins. According to the change in proteins’ structure and the interaction force, together with the the theoretical calculation and simulation, we attempt to conclude the mechanism on the interaction between particles and proteins; 2) we plan to monitor the nucleation and fibrillation process of the amyloid proteins was monitored using a combination of electron microscopy and spectroscopic techniques. We also investigate the effect of particles with different characteristics on the aggregation behavior of amyloid proteins. Furthermore, we try to understand the mechanism of amyloid aggregation in the presence of particles; 3) we plan to develop the new approaches for enhancing the desorption of particles from protein surface, inhibiting the amyloid aggregation or inducing the disaggregation of amyloid fibrils, aiming to clear the toxicity of particles. The methodologies and results of this proposal will provide a useful reference for researchers who wish to in-depth understand the biological effect and the toxicity mechanism of particles.