朱砂叶螨对杀螨剂代谢能力增强是其产生抗药性的重要机制。目前商品化甲氰菊酯都是以外消旋体的形式存在（R-甲氰菊酯：S-甲氰菊酯为1:1），因此得到的抗性机制都是两个对映体共同作用的结果。而有关朱砂叶螨对甲氰菊酯不同对映体代谢行为差异以及代谢差异与代谢抗性形成机制并不清楚。鉴于此，本项目在前期已证实甲氰菊酯对映体对朱砂叶螨毒力显著差异的基础上，将对映体区别对待，系统研究朱砂叶螨对甲氰菊酯不同对映体敏感性及抗性进化速率差异；结合质谱分析，明确对映体在朱砂叶螨中代谢物、代谢途径及酶代动力学差异，并确定参与对映体解毒代谢的关键酶；最后探明不同对映体胁迫后朱砂叶螨代谢抗性相关基因表达差异及代谢酶活性变化与抗性形成的关系。最终从对映体的独特视角阐明朱砂叶螨对甲氰菊酯对映体代谢差异及对映体代谢抗性形成机制，为全面了解和评估手性杀螨剂抗性风险、制订完善的抗性风险评估方案和正确的抗性治理策略提供科学依据。

The increased capability to metabolize acaricides is a vital mechanism of Tetranychus cinnabarinus resistance. At present, the commercial fenpropathrin is racemate with R-fenpropathrin and S-fenpropathrin at ratio of 1:1, thus all the obtained resistance mechanisms of fenpropathrin are the combined effects of R-fenpropathrin and S-fenpropathrin. However, few studies have been conducted to investigate the metabolic differences of fenpropathrin enantiomers as well as the relationship between enantioselective metabolism and the development of enantiomer metabolic resistance. Our previous study revealed the toxicity of R-fenpropathrin and S-fenpropathrin to T. cinnabarinus is significantly different. Therefore, this proposal will treat R-fenpropathrin and S-fenpropathrin as two different compounds and investigate the sensitivity difference and evolution rate of resistance difference of T. cinnabarinus to R-fenpropathrin and S-fenpropathrin, individually. Secondly, the structures of fenpropathrin enantiomer metabolites will be identified based on LC-QTOF and LC-MS/MS methods. The metabolites, metabolic pathways and pharmacokinetics parameters differences will compared between R-fenpropathrin and S-fenpropathrin. In addition, the key detoxifying enzymes that involved in fenpropathrin metabolism in T. cinnabarinus will be identified through enzyme inhibition study. Lastly, the differential gene expression will be compared with RNA-Seq deep sequencing and digital gene expression after S-fenpropathrin and R-fenpropathrin stress individually. Furthermore, the relationship between metabolic enzyme activity and evolution of resistance will also be identified after fenpropathrin enantiomer stress. Combining all the mentioned research results, the metabolic differences and enantioselective evolution of metabolic resistance in T. cinnabarinus will be clarified at enantiomer level. Such results will contribute to clarifying the metabolic resistance mechanism of T. cinnabarinus and providing a scientific basis for T. cinnabarinus resistance risk assessment and resistance management.