稻瘟菌是研究植物病原真菌致病机理的模式物种之一，但目前关于剪切因子在其致病性与生长发育中作用的报道很少。前期研究结果表明：剪切体的Prp19复合体的大部分组分在真核生物中是保守共有的，但稻瘟菌和其它高等真核生物的Prp19复合体具有低等真核生物如出芽酵母的Prp19复合体不存在的组分，称之为Prp19复合体多余组分。本项目拟对稻瘟菌中Prp19复合体的4个多余组分在其致病性和生长发育中的作用，及不同多余组分调控稻瘟菌致病性和生长发育的机理进行研究，内容包括：解析各多余组分在稻瘟菌致病性和生长发育中的作用、表达动态及互作蛋白；解析各多余组分调控的靶标内含子及对应的基因；分析比较各多余组分调控内含子的异同，对内含子进行分类并挖掘其序列特征。本项目结果不但会从新的角度来解析植物病原真菌的致病机理，而且可为动植物中Prp19复合体同源物的功能研究提供参考。

The rice blast fungus, Pyricularia oryzae, is one of the models for investigating plant fungal pathogenesis. But roles of splicing factors in fungal pathogenicity and development were seldom reported until now. Previously, the applicant found that most components of the Prp19-associated complex of the spliceosome are conserved and common among eukaryotes, but the Prp19-associated complex in P. oryzae and other higher eukaryotes contain components that do not exist in lower eukaryotes including the budding yeast, and called them as extra components of the Prp19-associated complex. In this project, the applicant will investigate roles of four extra components of the Prp19-associated complex in P. oryzae in fungal pathogenicity and development, and mechanism of different extra components regulating fungal pathogenicity and development, including: 1) analyzing roles of the four extra components in fungal pathogenicity and development, gene expression patterns, and their interacting proteins; 2) analyzing target introns and the corresponding genes controlled by the four extra components; 3) comparing and classifying the introns regulated by different extra components, and summarizing sequence features of the introns. Overall, this project will not only illustrate plant fungal pathogenesis from a new angle but also provide references for investigating roles and mechanisms of components of the Prp19-associated complex in animals and plants.