稻瘟病是在世界各水稻产区广泛发生、危害极大的真菌性病害，也是许多植物病理工作者研究的模式系统。深入地解析稻瘟菌的致病机理有助于发现控制稻瘟病及其它真菌病害的新途径。尽管已有许多研究从不同的角度来解析稻瘟菌的致病机理，但是关于基因转录后的调控过程在稻瘟菌致病中所起作用的研究还很缺乏。在前期工作中，本课题组从稻瘟菌中分离鉴定了一个致病突变体，在该突变体中一个编码含多个KH结构域蛋白的基因PCG6被破坏。本课题组已通过基因敲除和基因互补确认PCG6在稻瘟菌致病中发挥重要的作用，并发现PCG6负调控谷氨酸合成酶基因的表达。PCG6编码的蛋白推测通过调控靶标基因mRNA的稳定性起作用。本课题研究拟通过分子遗传学等方法，揭示PCG6在稻瘟菌中是通过调控哪些靶标基因mRNA的稳定性来参与致病性以及PCG6调控谷氨酸代谢的机理。本研究将为揭示植物病原真菌致病性与基因转录后调控之间的关系提供新的证据。

Rice blast is a widely distributed and devastating fungal disease on rice throughout the world and is a model system that is widely used for studying fungus-plant interactions. Deciphering the pathogenesis of the rice blast fungus will contribute to designing novel strategys for controlling the rice blast and many other fungal diseases. Though many studies have been carried out to investigate the molecular mechanism on pathogenesis in M. oryzae from diverse aspects, studies are lacked on the roles of post-transcriptional gene control in pathogenesis. Previously, we isolated an insertion mutant of M. oryzae that was defected in pathogenicity, in which a gene named PCG6 was disrupted. With the gene knockout and complementation, we confirmed that PCG6 is important for pathogenesis and found that PCG6 negatively regulates the expression level of the glutamate synthase gene GLT1. According to the primary structure, Pcg6 is a multiple-KH-domain-containing protein and may be invovled in regulating mRNA stability. In this study, we plan to integrate the approches of molecular genetics, molecular biology and bioinformatics, to decipher which genes are targets regulated by PCG6 and how PCG6 regulates glutamic acid metabolism. Our study will provide novel evidences for elucidating how the post-transcriptional gene control is involved in the pathogenesis of plant fungal pathogens.