阿姆斯特丹散囊菌（Eurotium amstelodami）是中国传统发酵体系中广泛分布的优势菌种，对发酵产品风味前体物质和独特品质形成起着重要作用。基因组中存在大量功能未知的沉默生物合成基因簇，在特定条件下激活，有望产生多种新化合物。本项目拟采用共培养和超表达LaeA策略激活沉默基因簇，HPLC指纹图谱叠加比对，分析各样品化合物的差异及新化合物的产生，色谱学和波谱学技术进行纯化与结构鉴定；RNA-seq与定量PCR初步解析新化合物的生物合成途径。本课题实施，可明确沉默次生代谢生物合成基因与新化合物之间的关联性，解析生物合成途径及调控机制，进一步确证阿姆斯特丹散囊菌的安全性，指导传统发酵产业及相关保健医疗产品开发。

Eurotium amstelodami, a member of core microbiota in traditional solid state fermentation, play important role in production of flavor components and quality of fermented foods, which have a large number of unknown silent biosynthetic gene clusters in genome. Novel bioactive compounds could be discovered using established methods to activate these silent gene clusters. In this study, we activate these silent gene clusters by microorganism co-culture and overexpression of LaeA, search the main variant phytochemistry and discovery of novel bioactive compounds by comparative analysis on HPLC fingerprints. Meanwhile, targeted variant compounds have been constructed and identified using chromatography and spectroscopy methods. The biosynthesis pathway of novel bioactive compounds were described using RNA-seq and real time PCR. These results would discover the relationship between silent gene cluster and novel compound, also illuminate the biosynthesis pathway of novel bioactive compounds and mechanism of regulation, and provide new insight into the safety of E. amstelodami, and promote the development of traditional food fermentation and health care product.