Overexpression of llm1 Affects the Synthesis of Secondary Metabolites of Aspergillus cristatus.
Overexpression of llm1 Affects the Synthesis of Secondary Metabolites of Aspergillus cristatus.
复制标题
llm1过表达影响冠曲霉次生代谢产物的合成
DOI：
10.3390/microorganisms10091707
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发表时间：
2022-08-24
期刊：
影响因子：
4.5
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Wang Y;Chen Y;Zhang J;Zhang C
推测的甲基转移酶被认为参与丝状真菌次级代谢产物的调节。在这里，我们报告了预测的LaeA-样甲基转移酶基因llm 1的过表达对鸡冠曲霉次生代谢产物合成的影响。我们的研究结果表明，llm 1基因在A.鸡冠状孢子显著阻碍分生孢子的产生，促进性发育，并降低对过氧化氢的氧化耐受性。与野生型相比，超表达突变体的代谢特征明显，多种次生代谢产物的含量显著增加，主要包括萜类和黄酮类化合物，如（S）-齐墩果酸、赤霉素A62、赤霉素A95、卵利烯酮、PD 98059和1-异芒果苷。利用转录组测序共鉴定出600个显著差异表达基因（DEG），并且DEG主要富集在跨膜转运和次级代谢相关的生物过程中。综上所述，过表达全局次级代谢物调节因子的策略成功激活了次级代谢物基因簇的表达，并且在A.鸡冠。该研究为深入开发利用黄芪新的次生代谢产物提供了新的思路。鸡冠。
Putative methyltransferases are thought to be involved in the regulation of secondary metabolites in filamentous fungi. Here, we report the effects of overexpression of a predicted LaeA-like methyltransferase gene llm1 on the synthesis of secondary metabolites in Aspergillus cristatus. Our results revealed that overexpression of the gene llm1 in A. cristatus significantly hindered the production of conidia and enhanced sexual development, and reduced oxidative tolerance to hydrogen peroxide. Compared with the wild-type, the metabolic profile of the overexpression transformant was distinct, and the contents of multiple secondary metabolites were markedly increased, mainly including terpenoids and flavonoids, such as (S)-olEuropeic acid, gibberellin A62, gibberellin A95, ovalitenone, PD 98059, and 1-isomangostin. A total of 600 significantly differentially expressed genes (DEGs) were identified utilizing transcriptome sequencing, and the DEGs were predominantly enriched in transmembrane transport and secondary metabolism-related biological processes. In summary, the strategy of overexpressing global secondary metabolite regulators successfully activated the expression of secondary metabolite gene clusters, and the numerous secondary metabolites were greatly strengthened in A. cristatus. This study provides new insights into the in-depth exploitation and utilization of novel secondary metabolites of A. cristatus.
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