共生菌CLS-Hl在长角血蜱生殖中的作用及其机制

蜱类为专性吸血的外寄生动物，具有较强的繁殖力，可传播病原体导致疾病，危害人类健康和畜牧业生产。共生菌在蜱类生物学和病原体的传播等方面均有重要作用，其中类考克次氏体是多种蜱的专性共生菌。项目组前期在长角血蜱Haemaphysalis longicornis中发现一种新的类考克次氏体，命名为CLS-Hl，并初步证实其显著影响宿主的生殖。但是，其作用机制并不清楚。在此基础上，本项目拟对CLS-Hl影响长角血蜱生殖的机制进行系统研究，以阐明CLS-Hl在卵巢组织中的分布和密度变化；揭示CLS-Hl对卵巢发育和细胞凋亡的影响；明确CLS-Hl对卵黄发生的影响。研究结果将明确CLS-Hl在长角血蜱生殖中的作用及其机制，为开展防治工作提供新思路。

As obligate blood-feeding ectoparasite, ticks exhibit extraordinary reproductive capability, and transmit a great variety of pathogens, which cause great damages to human health and livestock production. Symbiotic bacterium plays an important role in tick biology and pathogen transmission, and the Coxielle-like symbiont is obligate symbiont in many tick species. Our previous studies identified a new Coxielle-like symbiont (CLS-Hl) in Haemaphysalis longicornis, this symbiont significantly affects its host's reproduction. However, the underlying mechanism remains unclear. This study intends to systematically investigate the mechanism of CLS-Hl regulating the reproduction of H. longicornis. This study will clarify the distribution and density changes of CLS-Hl in ovarian tissue, identify the role of CLS-Hl on ovarian development and apoptosis, and clear the influence of CLS-Hl on host vitellogenesis. These results can reveal the function and mechanism of CLS-Hl in reproduction of H. longicornis and provide new ideas for ticks control.