家蚕microRNAs在家蚕与BmNPV互作机制中的功能研究

家蚕核型多角体病毒病是危害养蚕业的主要病原之一。迄今为止，对家蚕抗病毒的分子机理以及家蚕与病毒的互作机制知之甚少。研究表明microRNA广泛参与包括生长发育、细胞凋亡及抗病毒等一系列重要的生命过程。宿主编码的microRNA可通过靶向作用于病毒基因及自身基因在宿主与病毒的互作机制中发挥重要作用。因此，本研究旨在筛选与鉴定家蚕编码的与家蚕核型多角体病毒感染相关的microRNAs，探索部分microRNAs及其作用的靶基因在BmNPV与家蚕互作机制中的功能，为从分子水平上解析家蚕核型多角体病毒病的致病机理及免疫机制提供新的理论依据。

Bombyx mori nucleopolyhedrovirus(BmNPV), one of the major pathogens, greatly threaten the sericultural industry. Until now, little has been known on antiviral molecular mechanism and host-virus interaction in silkworm. Recently, accumulating reports reveal that microRNAs widely participate in several crucial biological processes including development, cell apoptosis and antiviral responses. MicroRNA from the host can play an important role in host-virus interaction by targeting the host genes as well as the virus genes. Therefore, this study aims to screen and identify the BmNPV-infected associated microRNAs from silkworm and explore the function of those microRNAs and targeted genes on silkworm-virus interaction and provide the new theoretic evidence for demonstrating pathogenic and antivirus immunity mechanism of BmNPV infection.