mRNA surveillance complex PELOTA-HBS1 regulates phosphoinositide-dependent protein kinase1 and plant growth.
mRNA surveillance complex PELOTA-HBS1 regulates phosphoinositide-dependent protein kinase1 and plant growth.
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mRNA监视复合体PELOTA-HBS1调控磷酸肌醇依赖性蛋白激酶e1和植物生长。
DOI：
10.1093/plphys/kiab199
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发表时间：
2021-08-03
期刊：
影响因子：
7.4
通讯作者：
Xue HW
中科院分区：
文献类型：
--
作者：
Kong W;Tan S;Zhao Q;Lin DL;Xu ZH;Friml J;Xue HW
在拟南芥中，PELOTA-HBS1 复合物调节 mRNA 监视和 3'-磷酸肌醇依赖性蛋白激酶 1 介导的植物生长和发育。信使 RNA (mRNA) 的质量控制系统是真核生物细胞活动的基础。为了阐明 3'-磷酸肌醇依赖性蛋白激酶 1 (PDK1)（在真核生物生长和发育过程中至关重要的主调节因子）的分子机制，我们采用正向遗传方法筛选拟南芥中功能丧失的 T-DNA 插入双突变体 pdk1.1 pdk1.2 的抑制子。值得注意的是，pdk1.1 pdk1.2 的严重生长衰减被 sop21（pdk1.1 pdk1.2 的抑制子）所挽救，sop21 含有 PELOTA1 (PEL1) 的功能丧失突变。 PEL1 是哺乳动物 PELOTA 和酵母（酿酒酵母）DOM34p 的同源物，它们各自与 GTPase HBS1（HSP70 SUBFAMILY B SUPPRESSOR1，也称为 SUPERKILLER PROTEIN7、SKI7）形成异二聚体复合物，GTPase HBS1 是一种负责核糖体救援的蛋白质，从而确保翻译过程中 mRNA 分子的质量和保真度。遗传分析进一步表明，功能失调的 PEL1-HBS1 复合物无法降解 T-DNA 破坏的 PDK1 转录本，这些转录本被截短但具有功能，从而挽救了 pdk1.1 pdk1.2 的生长和发育缺陷。我们的研究证明了同源 PELOTA-HBS1 复合物的功能，并确定了其在植物中的重要调节作用，为 mRNA 质量控制机制提供了见解。
In Arabidopsis thaliana, the PELOTA–HBS1 complex regulates mRNA surveillance and 3′-phosphoinositide-dependent Protein Kinase1-mediated plant growth and development. The quality control system for messenger RNA (mRNA) is fundamental for cellular activities in eukaryotes. To elucidate the molecular mechanism of 3′-phosphoinositide-dependent protein kinase1 (PDK1), a master regulator that is essential throughout eukaryotic growth and development, we employed a forward genetic approach to screen for suppressors of the loss-of-function T-DNA insertion double mutant pdk1.1 pdk1.2 in Arabidopsis thaliana. Notably, the severe growth attenuation of pdk1.1 pdk1.2 was rescued by sop21 (suppressor of pdk1.1 pdk1.2), which harbors a loss-of-function mutation in PELOTA1 (PEL1). PEL1 is a homolog of mammalian PELOTA and yeast (Saccharomyces cerevisiae) DOM34p, which each form a heterodimeric complex with the GTPase HBS1 (HSP70 SUBFAMILY B SUPPRESSOR1, also called SUPERKILLER PROTEIN7, SKI7), a protein that is responsible for ribosomal rescue and thereby assures the quality and fidelity of mRNA molecules during translation. Genetic analysis further revealed that a dysfunctional PEL1–HBS1 complex failed to degrade the T-DNA-disrupted PDK1 transcripts, which were truncated but functional, and thus rescued the growth and developmental defects of pdk1.1 pdk1.2. Our studies demonstrated the functionality of a homologous PELOTA–HBS1 complex and identified its essential regulatory role in plants, providing insights into the mechanism of mRNA quality control.
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影响因子：
3.5
作者：
Ferrari, Simone;Galletti, Roberta;De Lorenzo, Giulia
通讯作者：
De Lorenzo, Giulia
影响因子：
9.2
作者：
Casamayor, A;Torrance, PD;Alessi, DR
通讯作者：
Alessi, DR
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Furihata, T;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
6.7
作者：
Camehl I;Drzewiecki C;Vadassery J;Shahollari B;Sherameti I;Forzani C;Munnik T;Hirt H;Oelmüller R
通讯作者：
Oelmüller R
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR