橡胶树胶孢炭疽菌效应蛋白CgBAS的致病机制研究

胶孢炭疽菌引起的橡胶树炭疽病是造成海南橡胶产业减产的主要因素之一。效应蛋白在病原菌对寄主植物的侵染和病害发生中起着重要作用。效应蛋白的研究是当前国际植物病理学领域的研究热点。本课题组对一个强致病胶孢炭疽菌进行基因组测序，利用比较基因组学方法发现了含有两个成员的CgBAS效应蛋白家族。其中CgBAS2的缺失突变株表现为致病力显著下降且丧失产生分生孢子的能力。本项目计划通过筛选CgBAS在胶孢炭疽菌中的互作蛋白来研究CgBAS调控其生长和孢子产生等生理功能的机制；通过橡胶树原生质体瞬时表达系统研究CgBAS在植物细胞中的互作因子及其功能。以上研究结果将全面解析效应蛋白CgBAS在胶孢炭疽菌致病中的功能，对于建立新型病害防控策略具有理论意义。本项目从病原菌和寄主植物两个层次解析CgBAS的致病机制，同时将原生质体瞬时表达系统用于橡胶树病理学研究，在研究思路和研究方法上具有创新性。

The rubber anthrax caused by Colletotrichum gloeosporioides has brought serious economic loss to the rubber industry of Hainan. Recent researches showed that the effector proteins play important roles in the invasion and colonization of pathogen in host plant. Functions of effector have become a research focus to understand the mechanism of pathogenicity in the international field of plant pathology. Previously, we have predicted the effector protein family CgBAS (which contains two members, CgBAS2 and CgBAS4) of C. gloeosporioides by genome sequencing and bioinformatics. The growth rate and the pathogenicity of the CgBAS2 knockout mutant were seriously impaired, and the mutant lost the ability to generate conidia. In this project we are going to analyze the proteins regulated by CgBAS and their functions in regulating growth and conidiation of C. gloeosporioides. Secondly, we would isolate the targets of the CgBAS in the host cell and research the functions of the isolated targets by using the rubber protoplast transient expression system. This project will not only provide the new evidence for the molecular mechanism of CgBAS in regulating the pathogenicity of C. gloeosporioides, but also is helpful to build up new control strategy on rubber anthrax. The innovativeness of this project is that the pathogenic mechanism of CgBAS will be studied in both aspects of the pathogen and the host cell, based on using protoplast transient expression system on pathology of rubber tree.