柑橘酸腐病在果实贮藏保鲜过程中危害严重，且呈现逐年上升的趋势，这就极大地制约了柑橘贮藏保鲜的发展，因此研究酸腐病菌的致病调控机制是一个值得关注的科学问题。酸腐病菌对柑橘果实的成功侵染，涉及众多而且复杂的致病机制，但主要涉及的致病因子包括产生的酶类、毒素、酸性物质等，而致病基因在其发挥重要的作用。本课题组经in vivo柑橘果实接种实验筛选获得一株丧失致病能力的酸腐病菌突变体AY-1-66，但该突变体为什么不致病？以及它不致病的调控机制如何？目前还知之甚少。为此，本项目拟通过致病力分析比较柑橘酸腐病菌及突变体AY-1-66在细胞壁降解酶等的变化、葡萄糖酸等酸性物质的产生以及毒素类次生代谢物的含量，并通过DNA-蛋白质和蛋白质互作等手段，明确致病基因的靶基因、互作蛋白以及生物学功能，进而对致病基因参与柑橘酸腐病致病的分子机制进行全面的解析。研究结果将为生产上防控柑橘酸腐病提供新的重要理论基础。

It can be dangerous for the infection of sour rot during storage and keep-freshing in citrus, and it showed a rising trend year by year. Thus, it is a noteworthy scientific topic to explore the pathogenic mechanisms of sour rot in citrus. It involves many and complex pathogenic mechanisms, when the citrus fruit are successfully infected by sour rot. but the main pathogenic factors are involved the production of enzymes, toxins, and acidic substances, and the pathogenic genes play an important role in it. One strain with loss of pathogenic ability in T-DNA insertional mutant AY-1-66 from sour rot in citrus was screened by in vivo experiment, but why doesn't the mutant cause the disease? And the regulatory mechanism? It is remained largely unknown. Difference of sour rot and its mutant AY-1-66 was studied in the release of cell wall degradation enzyme, gluconic acid production, and toxins secondary metabolites. By means of DNA-protein and protein interaction, the target genes, mutual protein, and biological functions of pathogenic genes are clearly defined. And the molecular mechanism of pathogenic genes involved in citrus sour rot was comprehensively analyzed. The results obtained by this project will provide a new and important theoretical basis for preventing and controlling citrus sour rot.