环境内分泌干扰物EE2在农田根际土壤-作物系统中的环境行为及其微生物-植物跨界信号分子传导机制

EE2是一类典型EEDCs，农田土壤EE2污染将直接危害作物生长与健康，并通过食物链危害人体和动物健康，但目前国内外鲜见农田土壤和作物EE2的污染调查和转化归趋的报道。此外，群体感应(QS)和微生物-植物跨界信号传导在EE2迁移转化中的作用尚不清楚。本项目拟在探清EE2在土壤-农作物中的转归规律基础上，着眼于土壤污染—微生物降解转化—植物吸收代谢这一复杂过程，筛选耐受/降解EE2的优势根际细菌与根系内生菌，通过菌株互作阐明耐受/降解菌在作物根部定殖的生理机制，阐明QS对EE2在土壤-作物转归中的作用；联合根际、内生菌与植物共同作用，通过QS与植物信号分子分泌规律及其相互作用方式、以及Lux调控蛋白基因表达水平的研究，阐明EE2环境转归过程中植物-微生物间的信号分子传导机制。从根际菌与内生菌强化污染物降解角度为农田EEDCs的生态风险控制提供思路。

EE2 is a typical kind of environmental endocrine disruptor (EEDCs). The pollution of EE2 in the farmland soil will directly harm the growth and health of crops, and threat the health of human and animals through food chains. However, the information about the pollution investigation and their transformation and fate in the farmland soil and crops is limited. Additionally, the role of the microbial Quorum sensing (QS) and microbe-plant inter-kingdom signaling for the pollutants’ migration and transformation in soils is not clear. Therefore, in is project, the rule of transformation and fate of EE2 in farmland soil and crops will be explored; then, focusing on the complex process of the soil pollution-microbial degradation-plants’ absorb and metabolism, the dominant tolerable or degradable rhizosphere bacteria and root endophytic bacteria strains will be screened; by researching on the physiological mechanism of colonization of the tolerable or degradable bacteria in crop root, the effect of microbial QS on the transformation and fate of EE2 in the rhizosphere soil-crop system can be clarified; Finally, in combination of the effects of rhizosphere bacteria, endophytes and plant, by studying on the secretory regularity and modes of the interaction of the QS signal molecules and the signaling molecules in plants, and the gene expression level of Lux regulatory protein, the mechanism of the inter-kingdom signaling between microbe and plant will be elucidated. Above all, based on the enhanced degradation of pollutants by the rhizosphere bacteria and root endophyte, this project will provide new ideas for ecological risks’ control of the field EEDCs.