本研究是前国家自然科学专项基金项目的继续与深入，前期研究已证实脂质代谢紊乱是激素诱导股骨头坏死机制中的重要环节；中药骨蚀灵胶囊通过调节脂质代谢可有效地预防激素性股骨头坏死，但其作用机制尚不清楚。骨蚀灵是如何调控脂质代谢、主要影响哪些脂质代谢生物标志物以及与脂质代谢相关基因表达的关系还有待进一步探讨。因此，本项目拟基于脂质代谢组学理论，以超高效液相色谱-飞行时间质谱（UPLC-TOF/MS）为核心技术,以主成分分析法（PCA）和偏最小二乘法-判别分析(PLS-DA)为数据解析主要手段，建立激素诱导股骨头坏死干预前后的生物体代谢轮廓谱，通过比较其差异变化及特定靶标的定性、定量分析，确定能够表征激素性股骨头坏死发生、发展和评价中药骨蚀灵干预药效的潜在生物标记物；并通过与脂质代谢基因mRNA表达的整合生物信息学分析，从生物体内代谢层面的不同层次、不同深度阐释骨蚀灵干预激素诱导股骨头坏死的作用机制。

The study is further research based on a previous project funding by the Special Funds of the National Natural Science Foundation of China. In previous research, disorder of lipid metabolism was confirmed to be one of the important links about the mechanism of femoral head necrosis, which is induced by hormone. And Gushiling (GSL), one kind of traditional Chinese medicine, also has been provided to have excellent efficacy for prevention the glucocorticoid-induced femoral head necrosis via lipid metabolism. But the detailed mechanism is still unclear. There are still some problems need to be explored, such as how to regulate lipid metabolism of GSL, Which biomarkers in lipid metabolism are mainly affected by GSL, what kind of correlation between the biomarkers and the gene expressions related with lipid metabolism. Therefore, based on the theory of lipid metabonomics, metabolic profiling spectrum will be built using UPLC-TOF/MS in this project, and then PCA and PLS-DA will be used as main data analysis methods. Through the difference between before and after GSL treatment with femoral head necrosis were explored and specific targets were qualitative and quantitative analyzed, the potential biomarkers will be determined and applied in the characterization of the occurrence and development of femoral head necrosis and the evaluation of GSL efficacy. Finally, integrated bioinformatics analysis is carried out in the lipid metabolism biomarkers and related-gene expression data to elucidate the mechanism of GSL treatment glucocorticoid-induced femoral head necrosis, from different levels and depth in biological metabolism.