本研究是前国家自然科学基金项目的继续与深入。以往研究证实脑内微环境是脑瘫后大脑神经再塑与功能重建的关键，脑内微环境中的细胞因子、神经递质在神经干细胞的增殖与分化中扮演重要角色。然而，作为基因组、转录组及蛋白组的“终端”-代谢生物标记物是否同样影响脑内微环境及大脑神经的再生与重建？目前尚无确证。这对从生物体代谢的更深层面探讨脑瘫病理生理特点及康复机制具有重要的意义。本课题拟以脑瘫鼠模型和具有脑瘫高危因素的新生儿为研究对象，以头针疗法为干预手段，以脑内微环境为切入点，基于超高效液相色谱-飞行时间质谱（UPLC/TOF MS）的代谢组学技术分析，建立脑瘫及不同头针疗法干预下的生物代谢轮廓谱，通过比较其差异变化及特定靶标的定性、定量分析，确证能够表征脑瘫病理生理状态和评价不同头针疗法干预效果的潜在生物标记物，从生物体代谢的更深层面阐释脑瘫的病理生理特点及头针疗法促进脑瘫康复的作用机制。

The research is a persistent and in-depth study on the basis of the former Natural Science Foundation project. Previous research has confirmed that the brain micoenvironment is the key to regeneration of the nervous system and reconstruction of the brain founction.Both cytokines and neurotransmitters play the important role in the process of the replication and proliferation of neural stem cells. However, Will metabolic biomarkers affect brain microenvironment and the regeneration and reconstruction of nervous system as a genome, transcription and protein group "terminal"? which is of great significance to explore characteristics of cerebral palsy pathology ,physiology and rehabilization mechanism based on the biological metabolic level even though it hasn’t yet been confirmed. The topic will take the model of cerebral palsy rats and the neonatal children as the research object that have risk factors of cerebral palsy , use the scalp acupuncture therapy for intervention, take the brain microenviroment as the breakthrough point, set up the metabolism fingerprint spectrum of both cerebral palsy and under different the scalp acupunture intervention based on UPLC/TOF MS metabolomics analysis technology, by comparing its difference of changes and qualitative and quantitative analysis of specfic targets so as to prove potential biomarkers that can be characterization of ocurrence and development of cerebral palsy and evaluation of head acupuncture intervention effect, interpret the characteristics of cerebral palsy pathology ,physiology as well as the mechanism of scalp acupuncture therapy promoting cerebral palsy rehabilitation from deeper level.