磁场能改变蛋白分子的排列，从而影响细胞分裂、迁移等过程。蛋白分子只在缓冲盐溶液中才具有活性，受限于溶液、强磁场下蛋白单分子成像技术，磁场影响细胞生物学过程的内在机制依然模糊。STM虽能在溶液中观测蛋白分子结构，但受限于磁体的狭小口径、强磁场作用和震动干扰等困难，强磁场溶液STM尚未见报道。申请人已利用新设计的独立扫描单元，自主搭建了一套高稳定溶液STM，结合改进的“逐行恒高模式”第一次观测到EGFR蛋白的亚分子图像及其在0.4T磁场中的取向行为，并捕捉到纳米纤维的组装/解组装动态过程。本项目中，我们将利用新搭建的35T水冷磁体溶液STM，在0-35T下研究STK16和C-kit蛋白的磁场效应，在单分子尺度上给出强磁场影响STK16和C-kit蛋白构型、取向以及分子间相互作用的直观证据，阐明强磁场影响上述蛋白分子活性的内在机制，进而为探索强磁场下的细胞分裂、迁移等生物学过程奠定坚实基础。

The magnetic field can change the arrangement of protein molecules, thus affecting the cell division, migration and other life processes. Most biomolecules, especially for protein, function only in buffer salt solution, however, limited to the imaging technique of single protein molecule under solution and magnetic field, how magnetic field affect cell division and migration processes remains unclear. STM can be used to investigate the molecular structure of protein in solution, however, due to the limitations of the narrow bore size of magnet, STM was rarely combined with extreme conditions of solution and strong magnetic fields. So far, STM working in solution and strong magnetic field has not been developed yet. In the early, we have built and applied a high stable Liquid-phase STM to reveal the sub-molecular details of EGFR molecule and its orientation behavior under 0.4T static magnetic field. In addition, we have captured the assembly / disassembly dynamic process of nanofiber under solution conditions. In this project, we will use our new-built 35T water-cooled magnet STM investigating the magnetic behavior of STK16 and C-kit protein under physiological conditions, give the evidence of magnetic field-induced changes of protein structure, orientation and intermolecular interactions at single molecular level, illustrating the underlying mechanism of how magnetic field affect protein activity, building a foundation for exploring the biological processes of cell division and migration under magnetic field.