鼠疫的预防和控制是现阶段亟需解决的重要医学问题，对其宿主喜马拉雅旱獭的研究仅局限于传统形态分类、物种生态调查、动物鼠疫监测和个案分子研究。本项目拟在青海动物鼠疫流行猛烈地区，应用线粒体DNA基因和微卫星序列等多基因遗传分子标记技术，研究青海主要鼠疫源地内旱獭种群多样性和系统进化关系，分析不同旱獭地理种群在青藏高原的扩散过程，建立喜马拉雅旱獭线粒体DNA和微卫星序列数据库。并结合鼠疫菌的基因空间分布、人间鼠疫流行特点，综合探讨宿主遗传特征与病原体基因分型及人间鼠疫时空发展规律间的相互关系，为制定鼠疫预防控制措施、应急反恐、生物安全、软件技术平台体系建设提供科学依据，对进一步研究鼠疫致病机理和保存机制做出有益尝试。

Plague prevention and control should be solved as an important medical problem at this stage. The host of plague that Himalayan marmot is limited to the traditional morphological classification, species ecological surveys and monitoring of animal plague and few molecular studies. We studied on the population diversity and phylogenetic relationship and analyzed population diffusion process by the multi-gene markers technology used mitochondrial DNA gene sequences and microsatellite genetic and established the Himalayan marmot gene databases of the main violent region of animal plague in Qinghai province. Adding to the genotype space of Yersinia pestis, combined with the morphological characteristics and genetic information and the human plague epidemic characteristics to explore the relationship host and pathogen genotyping and human plague, and provide a scientific basis on the control measures, emergency anti-terrorism, biosecurity, system of software platform, for the further study on pathogenesis and preservation mechanism.