马铃薯具有自身愈伤修复的特性，该特性在马铃薯受到机械伤害后的防御修复过程中所发挥的作用倍受关注，但针对鲜切马铃薯而言，如何有效控制愈伤修复，以保持其新鲜品质的研究未见报道。本项目利用细胞生物学、代谢组学等知识与手段，研究还原型Vc(AsA)抑制鲜切马铃薯愈伤组织形成及其调控机理。研究内容：1.研究AsA处理鲜切马铃薯对活性氧(ROS)诱导愈伤组织形成的影响，分析H2O2含量及其亚细胞定位；2.研究ROS代谢酶系CAT、POD、APX、GR、POD、NOX、SOD和苯丙烷代谢酶系PAL、C4H、3CH等基因表达与酶活性关系，筛选POD、NOX、SOD基因家族中调控ROS代谢的靶基因；3.利用靶向代谢组学技术，研究鲜切马铃薯愈伤组织中单体酚、聚合体酚和木质素的代谢通路及其调控，并对愈伤组织细胞结构进行表征，阐明H2O2介导下的POD基因家族在单体酚聚合形成二聚体和多聚体过体过程中所发挥作用。

Healing repair is a biological characteristic of potato, which has drawn much attention in the field of preventing mechanical injury and pest of potato. However, little research has been done on control the repair of the healing tissue and maintain the quality for fresh-cut potatoes. This project uses cell biology, metabolomics and other methods to study the inhibitory effects of L-ascorbic acid (AsA) on the formation and regulation mechanism of fresh-cut potato healing tissue. The major research content including: 1. The effect of AsA treatment on the formation of reactive oxygen species (ROS) inducing healing tissue in fresh-cut potato was investigated and the content of H2O2 and the subcellular localization of fresh-cut potato were analyzed; 2. The relationship between gene expression and enzyme activity on ROS metabolic enzymes (CAT, POD, APX, GR, POD, NOX, SOD) and phenylpropane metabolic enzymes (PAL, C4H, 3CH) were investigated, and the key target genes of POD, NOX, SOD gene family which regulating the ROS metabolism were screened; 3. Targeted metabolomics was used to study the metabolic pathways and regulation of monomeric phenol, polymer phenol and lignin in fresh-cut potato healing tissue and characterize the healing tissue cell structure to elucidate the role of H2O2-mediated POD gene family in the polymerization of monophenols to dimers and multimers.