泛素特异性蛋白酶22（USP22）在多种恶性肿瘤侵袭和转移过程中发挥重要作用。申请者在前期工作中发现microRNA-329（miR-329）在具有转移潜能肝癌细胞系中表达下调，并且USP22的表达受到miR-329调控。提示miR-329可能通过调控USP22进而影响肝细胞癌（HCC）侵袭和转移。本研究将进一步验证miR-329与USP22之间的相互作用，并确定作用位点；明确miR-329在HCC侵袭和转移中的作用；并利用Protein Pathway Array技术结合Ingenuity Pathway Analysis分析软件验证miR-329调控USP22对HCC侵袭和转移信号传导网络的影响，从信号传导通路角度阐明miR-329影响HCC侵袭和转移的机制。通过本研究有望阐明miR-329调控USP22在HCC侵袭和转移中的作用及机制，为建立相应的阻断途径提供理论依据及新的阻断靶点。

Ubiquitin-specific protease 22 (USP22) plays an important role in a variety of tumor's invasion and metastasis. In previous work, the applicant found that microRNA-329 (miR-329) levels was downregulated in hepatocellular carcinoma cell lines with metastatic potential. Moreover, USP22 expression is regulated by miR-329. It is reasonable to suggest that miR-329 could target USP22 to affect HCC's invasion and metastasis. This study will further validate the interaction between miR-329 and USP22, identify the sites of action. Clarify the effect of miR-329 in the process of HCC's invasion and metastasis. Verify the effect of miR-329 regulates the signal transduction pathway of HCC's invasion and metastasis by using the Protein Pathway Array technology combined with the Ingenuity Pathway Analysis software. Elucidate the mechanism of miR-329 affect HCC's invasion and metastasis from the perspective of the signal transduction pathway. It is expected to clarify the function of miR-329 targeting USP22 in invasion and metastasis of HCC and its mechanism through this study, which provide theoretical basis and new therapeutic targets for establishing corresponding blocking pathways.