长链非编码RNA LINC01615在肝细胞癌（HCC）转移中的作用机制尚不明确。申请者前期研究发现LINC01615在HMGA1蛋白辅助下，能够增强THBS2的表达；过表达的THBS2能够激活TGF-β通路活化下游转移相关基因从而促进HCC转移。基于此，我们推测LINC01615/HMGA1/THBS2/TGF-β通路在HCC转移过程中发挥关键的调控作用。本研究拟通过：体内外实验确认LINC01615在HCC转移中的作用；重点研究LINC01615-HMGA1组成复合体并促使启动子和增强子形成染色质环，继而增强THBS2表达，以及THBS2通过TGF-β通路活化下游基因的分子机制；转基因小鼠验证LINC01615功能及分子机制；分析LINC01615及其下游基因与HCC临床病理特征之间的相关性。本研究旨在探索HCC转移新机制，为寻找HCC更具特异性的转移监测方法和治疗靶点提供新的依据。

The mechanism of long-chain non-coding RNA LINC01615 in hepatocellular carcinoma (HCC) metastasis is unclear. Applicant's previous study found that LINC01615 could enhance the expression of THBS2 with the help of HMGA1 protein. Overexpression of THBS2 could activate TGF-β pathway to activate downstream metastasis-related genes and promote HCC metastasis. Based on this, we speculate that the LINC01615/HMGA1/THBS2/TGF-β pathway plays a key regulatory role in HCC metastasis. The following research should be carried out: confirm the role of LINC01615 in HCC metastasis in vitro and in vivo, and focus on the molecular mechanism of the composition of LINC01615-HMGA1 complex promoting the formation of chromatin loops by promoters and enhancers, which in turn enhance THBS2 expression, as well as THBS2 activating downstream genes via TGF-β pathway; Transgenic mice are used to verify the function and molecular mechanism of LINC01615; Analyze the correlation between LINC01615 and its downstream genes and clinicopathological features of HCC. The purpose of this study is to explore new mechanisms of HCC metastasis and to provide new proof for finding more specific metastatic monitoring methods and treatment targets.