以亚硝酸盐为电子受体氧化甲烷的亚硝酸盐型甲烷厌氧氧化（N-DAMO）是最近环境科学与工程领域的一个重大发现，以此为基础研发的N-DAMO工艺由于可实现对亚硝酸盐和甲烷两种污染物的同步去除而在环境工程领域具有很高的开发应用价值。但在前期的研究中发现，尽管采用无机培养基，获得的N-DAMO富集物中总伴生有反硝化菌，反硝化菌的存在降低了N-DAMO菌的富集度，并影响该工艺的脱氮除碳效果，目前对这类伴生菌没有相关研究。搞清楚反硝化菌和N-DAMO菌的伴生关系，阐明群落演替机理;在此基础上建立N-DAMO数学模型，利用模型优化反应器设计和运行，强化N-DAMO工艺并建立反应器运行评价和控制系统,为该工艺的工程化应用提供理论依据。搞清反硝化菌和N-DAMO菌的伴生关系，不仅可以丰富微生物学内容，也有助于该工艺的开发应用，推动新型低耗的厌氧生物脱氮除碳工艺同时减少温室气体N2O和甲烷的排放。

Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a newly discovered bioprocess, which is catalyzed by M.oxyfera bacteria that anaerobically oxidize methane coupled with nitrite reduction. N-DAMO is a potential process in environmental engineering for simultaneous removal of methane and nitrogenous compounds. However, the previous works show that it's not possible to eliminate denitrifying bacteria from the N-DAMO enrichment in spite of that inorganic medium was used. The survived denitrifying bacteria decrease the abundance of N-DAMO bacteria and impact the performance of process, and succeeded in escaping from the previous researchers' attention. In this work, it will study on the associated relationship between N-DAMO and denitrifying bacteria and the mechanism of community succession in the N-DAMO enrichment. On the basis of the previous results, an N-DAMO mathematical model will be developed to optimize the design and operation of N-DAMO reactor and to establish an evaluation and control system of N-DAMO reactor. This work will provide theoretical foundation for the engineering application of the novel N-DAMO process, and is also useful for reducing the emissions of greenhouse gases, methane and N2O, and expanding the knowledge of microbiology.