A novel denitrifying methanotroph of the NC10 phylum and its microcolony.

A novel denitrifying methanotroph of the NC10 phylum and its microcolony.
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NC10门的新型反硝化甲烷氧化菌及其小菌落

DOI:
10.1038/srep32241
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发表时间:
2016-09-01
期刊:
影响因子:
4.6
通讯作者:
Hu B
Hu B
中科院分区:
综合性期刊3区
文献类型:
--
作者:
He Z;Cai C;Wang J;Xu X;Zheng P;Jetten MS;Hu B

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NC 10门是原核生物的一个候选门,在地球化学循环和进化史中具有重要意义。NC 10成员是尚未培养的,很难丰富,我们对这个门的知识主要限于第一个种'Methylomirabilisoxyfera'(M。oxyfera)。在这里,我们丰富的NC 10成员从水稻土,并获得了一个新的物种的NC 10门介导的甲烷(AOM)厌氧氧化耦合到亚硝酸盐还原。通过与数据库中已有的16 S rRNA基因序列比较,发现该新种广泛分布于中国的各种生境。因此,我们暂将其命名为“中华野牡丹(Methylomirabilissinica)”。sinica)。M. sinica为球形(0.7-1.2 μm),与mmm不同。尖叶(杆状; 0.25-0.5 × 0.8-1.1 μm)。值得注意的是,显微镜检查显示,M. sinicagrew在蜂窝状小菌落中,这是NC 10门小菌落的首次发现。这一发现开启了使用微菌落依赖性分离策略分离NC 10成员的可能性。
The NC10 phylum is a candidate phylum of prokaryotes and is considered important in biogeochemical cycles and evolutionary history. NC10 members are as-yet-uncultured and are difficult to enrich and our knowledge regarding this phylum is largely limited to the first species ‘CandidatusMethylomirabilis oxyfera’ (M. oxyfera). Here, we enriched NC10 members from paddy soil and obtained a novel species of the NC10 phylum that mediates the anaerobic oxidation of methane (AOM) coupled to nitrite reduction. By comparing the new 16S rRNA gene sequences with those already in the database, this new species was found to be widely distributed in various habitats in China. Therefore, we tentatively named it ‘CandidatusMethylomirabilis sinica’ (M. sinica). Cells ofM. sinicaare roughly coccus-shaped (0.7–1.2 μm), distinct fromM. oxyfera(rod-shaped; 0.25–0.5 × 0.8–1.1 μm). Notably, microscopic inspections revealed thatM. sinicagrew in honeycomb-shaped microcolonies, which was the first discovery of microcolony of the NC10 phylum. This finding opens the possibility to isolate NC10 members using microcolony-dependent isolation strategies.
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