A novel denitrifying methanotroph of the NC10 phylum and its microcolony.
A novel denitrifying methanotroph of the NC10 phylum and its microcolony.
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NC10门的新型反硝化甲烷氧化菌及其小菌落
DOI:
10.1038/srep32241
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发表时间:
2016-09-01
影响因子:
4.6
通讯作者:
Hu B
中科院分区:
文献类型:
--
作者:
He Z;Cai C;Wang J;Xu X;Zheng P;Jetten MS;Hu B
The NC10 phylum is a candidate phylum of prokaryotes and is considered important in biogeochemical cycles and evolutionary history. NC10 members are as-yet-uncultured and are difficult to enrich and our knowledge regarding this phylum is largely limited to the first species ‘CandidatusMethylomirabilis oxyfera’ (M. oxyfera). Here, we enriched NC10 members from paddy soil and obtained a novel species of the NC10 phylum that mediates the anaerobic oxidation of methane (AOM) coupled to nitrite reduction. By comparing the new 16S rRNA gene sequences with those already in the database, this new species was found to be widely distributed in various habitats in China. Therefore, we tentatively named it ‘CandidatusMethylomirabilis sinica’ (M. sinica). Cells ofM. sinicaare roughly coccus-shaped (0.7–1.2 μm), distinct fromM. oxyfera(rod-shaped; 0.25–0.5 × 0.8–1.1 μm). Notably, microscopic inspections revealed thatM. sinicagrew in honeycomb-shaped microcolonies, which was the first discovery of microcolony of the NC10 phylum. This finding opens the possibility to isolate NC10 members using microcolony-dependent isolation strategies.
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影响因子:
4.4
作者:
KANE, MD;POULSEN, LK;STAHL, DA
通讯作者:
STAHL, DA
影响因子:
5.1
作者:
Fujitani, Hirotsugu;Ushiki, Norisuke;Aoi, Yoshiteru
通讯作者:
Aoi, Yoshiteru
影响因子:
11.4
作者:
He, Zhanfei;Cai, Chen;Hu, Baolan
通讯作者:
Hu, Baolan
DOI:
10.1073/pnas.1411617111
发表时间:
2014-12-23
影响因子:
11.1
作者:
Deutzmann, Joerg S.;Stief, Peter;Schink, Bernhard
通讯作者:
Schink, Bernhard
影响因子:
5
作者:
Chen, Jing;Zhou, Zhichao;Gu, Ji-Dong
通讯作者:
Gu, Ji-Dong