控制脐橙贮藏性能的关键果皮蜡质基因的分离和功能验证

脐橙果皮蜡质是覆盖在果实最外面的保护层，其果皮蜡质的厚度、形态结构、化学成分等都直接影响果实的贮藏性能。该项目选用本课题组独有的脐橙果皮光泽型蜡质突变体为材料，采用RNA-seq（定量）与蛋白质组同位素标记相对和绝对定量（iTRAQ）技术，在温度4℃不同时间冷藏条件下，比较纽荷尔脐橙（母本-对照）和光泽型突变体低温贮藏后基因表达的差异、蛋白质组差异，并对转录组和蛋白组数据进行关联分析，进一步筛选出脐橙耐低温贮藏的关键蜡质候选基因。结合本课题组已获得的该研究材料在贮藏期间蜡质形态结构变化特征、蜡质组成成分变化的代谢组数据, 及通过实时定量（RT-qPCR）和转基因技术鉴定关键蜡质候选基因的功能，从而最终鉴别出脐橙耐低温贮藏的关键蜡质基因。达到初步阐明脐橙采后果皮蜡质成分变化会影响脐橙果实低温贮藏性能的分子机制，为今后通过基因工程改良柑橘低温贮藏性能打下坚实的理论基础。

The fruit peel wax layer acts as a outmost obstacle to protect navel orange against biotic and abiotic stresses. Moreover, its thickness, morphology, and composition is closely related to the storage capability of navel orange. In this project, the gene expression and proteomic profile differences will be compared between 'Newhall'navel orange (control) and its particular'glossy Newhall'mutant fruit peels by RNA-seq（quantification）and iTRAQ after 4℃ storge. At the same time, the transcriptomic and proteomic profiling will be related to screen out the key wax biosynthesis and export candidate genes controlling the storage capability of navel orange. Afterwards, combined with metabolite data of different wax morphology and composition changes between control and mutant fruit during 4℃ storge which have been obtained and the RT-qPCR and transgenic technology, the key wax genes controlling the storage capability of navel orange will be finally identified.This project will illuminate the molecular mechanism of the navel orange fruit peel wax changes during cold storge controlling its storage capability and provide a solid theoretic base for improving the citrus cold storage capability.