控制脐橙果皮蜡质合成和运输的关键非编码RNAs的筛选和功能鉴定

脐橙果皮蜡质是覆盖在果实最外面的保护层，具有抵御生物和生物胁迫等重要的生理功能。本项目利用申请者培育的果皮光泽型蜡质芽变新品种“赣脐3号”和其母本品种“纽荷尔脐橙”为研究材料，运用高通量测序技术比较两材料果实发育过程中mRNAs和各类非编码RNAs（non-coding RNAs，ncRNAs）的表达谱差异，鉴定差异表达ncRNAs的靶基因，筛选调控脐橙蜡质合成与运输的关键ncRNAs。并通过实时定量PCR、RLM-5’RACE和转基因等技术验证关键蜡质ncRNAs的功能。最后，通过mRNA与ncRNAs的联合分析，构建ncRNAs控制脐橙果皮蜡质合成和运输的调控网络，从而从转录、转录后和翻译后等多个水平解析ncRNAs控制脐橙果皮蜡质合成和运输的分子机理。

The wax layer of fruit peel acts as an outmost obstacle to protect navel orange against biotic and abiotic stresses. In this project, the mRNAs and ncRNAs expression differences will be compared between the fruit peels of‘Newhall’navel orange and its bud mutant variety ‘Ganqi 3’ by Illumina sequencing. The target genes of differential expressional ncRNAs were identified to screen the ncRNAs related to wax biosynthesis and export of navel orange. The function of key wax-related ncRNAs were identified by Real-time quantitative PCR, RLM-5’RACE and transgenic technology. Finally, the ncRNAs-mRNAs network will be constructed by ncRNAs and mRNAs combined analysis to illuminate the molecular mechanism of wax biosynthesis and export in navel orange at the transcriptional, post transcriptional, and post-translational levels.