谷子是我国的特色作物，也是内蒙古自治区的主要杂粮作物。谷子品质对其产业化开发具有重要决定作用，选育优质高产糯谷子品种是谷子生产中的迫切要求。国内在谷子营养品质、食味品质、杂优利用、基因组测序与注释、waxy基因序列多态性等方面进行了大量研究，但未见有关胚乳形成期淀粉合成酶活性动态与转录组分析的报道。本项目以粳谷×糯谷及其杂交后代为材料，分析其灌浆过程中籽粒淀粉积累动态和淀粉合成酶活性的变化。利用高通量测序技术对其mRNA测序数据进行参考序列比对分析；预测分析新的转录本、剪切体和差异性SNP/InDel；评估RNA-seq整体质量，进行差异表达分析；对差异基因进行GO富集、KEGG富集和蛋白互作富集分析；利用荧光定量 RT-PCR检测差异表达基因的相对表达量，并与Illumina测序结果比较。旨在发掘谷子淀粉合成的代谢网络和一些淀粉合成的关键基因及功能，为谷子品质分子技术改良提供参考依据。

Foxtail millet is a special crop in China，and is main grain crop in the Inner Mongolia Autonomous Region. Millet quality plays an important decisive role in industrialization development. High quality and yield waxy millet cultivars are urged in millet production. Nutritional quality, eating quality, heterosis utilization, genome sequencing and comments, waxy gene polymorphisms were studied for millet, but not reported on dynamics of starch synthase activity and their transcriptome analysis in endosperm formation in China. Hybrid parents of no-waxy and waxy millets and their hybrids, which have been equipped by our research group, will be used to analyze the grain starch accumulation dynamic and their changes of starch synthase activity in the process of grouting. Alignment mRNA sequencing data to millet reference genome sequences by high-throughput transcriptome sequencing technology(RNA-seq). Predict new transcript, shear and different SNPs/InDel. Assessment advanced quality of RNA-seq and different express analysis. Different gene GO, KEGG and protein interaction enrichment will be conducted. Detect the relative expression of differentially expressed genes using fluorescence quantitative RT-PCR, which will be compared with Illumina sequencing results. Aimed at exploring metabolic networks of millet strach synthesis and some key genes and function in starch synthesis, providing experimental basis on millet quality improvement by molecular techniques.