Ghatp1基因RNA编辑在棉纤维细胞形态建成中的作用机制研究

研究棉纤维发育相关基因的功能和调控机制，对于改善棉纤维的品质和提高纤维产量具有重要的理论意义和应用价值。近年来研究转录后加工修饰现象与棉纤维细胞生长发育的关系已成为新一轮的热点问题。RNA编辑是一类新的转录后加工修饰现象，已有研究表明RNA编辑参与了植物生长发育的许多过程，但RNA 编辑在棉纤维形态建成过程中是否也具有重要作用，目前仍属未知。本课题的前期研究结果表明Ghatp1基因在伸长的棉花纤维细胞中会发生RNA编辑，且编辑的Ghatp1基因能恢复缺失atp1酵母的生长及能荷产生；此外，Ghatp1基因各编辑位点编辑效率的改变也与纤维发育有一致性。在此基础上，本课题将进一步利用酵母突变体、拟南芥突变体及棉花离体胚珠实验，深入了解Ghatp1发生RNA编辑的生物学意义及与纤维生长发育的关系，同时利用凝胶阻滞技术探讨Ghatp1发生RNA编辑的机制，以填补棉纤维生长发育过程中存在的理论空白。

To investigate the function and regulatory mechanisms of cotton fiber development-related genes has important academic significance and applied value for improving the quality of cotton fiber and increasing fiber yield. Recently years, research hotspot has focused on the relationship of post-transcriptional processing and modification with cotton fiber growth and development. RNA editing is a kind of new phenomena of post-transcriptional processing and modification. Some results have indicated RNA editing involves many aspects of plant development. But so far, it is still unknown whether RNA editing has any relationship with cell morphogenesis of cotton fiber. Our experimental results had shown that Ghatp1 gene has RNA editing phenomena in elongated fiber cells. Transfered edited Ghatp1 into yeast cell abscented atp1, the mutant yeast can restore to normality in morphogenesis and energy charge production. Moreover, the change of editing efficiency of Ghatp1 has consistence with fiber development. Based on the results, we will further explore the biological significance of edited Ghatp1 by using mutant complementation experiments of yeast and Arabidopsis, and vitro ovule transfer extraneous edited gene to uncover the relationship of cotton fiber development with RNA editing of Ghatp1. We also investigate the mechanisms of the occurrence of Ghatp1 editing via gel shift assay. All these results can help us to fill the theory blank during the process of cotton fiber development and elongation.