前期研究发现寡糖具有调节血糖作用，但与其降血糖相关的作用靶点尚不明确。目前正缺乏一种能迅速发现寡糖多重作用靶点的有效方法。因此，本项目以海藻酸钠寡糖（AOS）为研究对象，设计其作用靶点钓取体系，并探索其在活体动物和细胞层面的作用规律。主要研究内容有：（1）从化学生物学途径出发，对AOS进行分子探针标记；（2）将标记的AOS与聚赖氨酸荧光纳米球（S-F-PLys）交联，得到新体系（AOS-BFG/S-F-Plys），并对其降血糖活性和安全性进行评价；（3）观察AOS-BFG/S-F-Plys在活体动物和组织细胞定位成像信息及对应的靶点垂钓数据，富集并鉴定其对应的靶蛋白；通过多种技术分析靶蛋白与AOS之间的相互作用，根据它们的作用方式和强度来确定其作用靶点。本项目能够积累寡糖结构设计及其降糖靶蛋白识别方面的基础研究数据，有利于寡糖降血糖分子机制理论体系的完善，为新型寡糖功能食品的研发奠定基础。

Previous studies showed that oligosaccharides have a role in regulating blood sugar, but their hypoglycemic related multiple targets are unclear. An effective strategy is needed to rapidly discover their multi-targets. Therefore, the project is to use alginate oligosaccharides (AOS) as an research object, aiming at designing a new system to confirm multi-target of AOS and to explore rules in live animals and cells. The main contents are: (1) based on chemical biological pathways, AOS is marked by molecular probe forming AOS-BFG; (2) the AOS-BFG and poly lysine fluorescent nano ball (S-F-PLys) are crosslinked, forming a new system (AOS-BFG/S-F-Plys), and its hypoglycemic activity and safety are evaluated; (3) The AOS-BFG/S-F-Plys is employed in living animals and cells to achieve information on the AOS’s localization and corresponding targets “fishing” data, and the corresponding target proteins are further enriched and identificated. A variety of techniques are used to analyze the interaction between target proteins and the AOS, the promising targets are confirmed according to their action mode and strength. The results provide basic research data for structure design and recognition of target proteins of hypoglycemic effect of oligosaccharides, which helps to clarify the hypoglycemic mechanism of oligosaccharides and to develop functional foods of oligosaccharides.