有丝分裂纺锤体的定向通过控制组织形态发生，在高等生物的生长和发育中发挥重要作用。纺锤体定向的异常与肿瘤等多个重大疾病有密切的关系。目前已确定星体微管与细胞皮层之间的动态相互作用是纺锤体定向的核心。但是，关于纺锤体定向的精确机制，尤其该过程中星体微管动态性的调节及微管相关蛋白的行为，目前还了解得很不透彻。在本项目中，我们将通过研究OFIP（OFD1 and FOR20 interacting protein，又称为KIAA0753）对星体微管动态性的调节、对马达蛋白募集至细胞皮层的调节、与已知微管结合蛋白的相互作用等，来深入理解OFIP调节纺锤体定向的分子机制。我们还将利用OFIP的表皮特异性敲除小鼠，深入了解OFIP调节纺锤体定向在组织形态发生中的功能。这些研究有助于加深对纺锤体定向的分子细节和调控机理的了解，并有助于促进对纺锤体定向在组织器官发育中所发挥作用的认识。

By regulating tissue morphogenesis, orientation of the mitotic spindle is critical for the growth and development of higher organisms. Spindle misorientation is associated with cancer and many other human diseases. The dynamic interaction between astral microtubules and the cell cortex is known to play a key role in spindle orientation. However, the precise mechanisms of spindle orientation, especially the control of astral microtubule dynamics and the activity and behavior of microtubule-binding proteins in spindle orientation, remain elusive. In this project, we will study the molecular mechanisms underlying the regulation of spindle orientation by OFIP (OFD1 and FOR20 interacting protein, also known as KIAA0753), by investigating how this protein regulates astral microtubule dynamics, how it regulates the recruitment of motor proteins to the cell cortex, and how it interacts with known microtubule-binding proteins. We will also use epidermis-specific OFIP knockout mice to study the role of OFIP-dependent spindle orientation in tissue morphogenesis. These studies will help to elucidate the molecular details and regulatory mechanisms of spindle orientation. In addition, these studies will help to better understand the functions of spindle orientation in the development of tissues and organs.