EB1/CYLD互作对微管动态性及细胞分裂和迁移的调控

我们近期研究发现微管正端跟踪蛋白EB1与新型微管结合蛋白CYLD相互作用，并发现二者的相互作用可能在微管动态性的调控及微管依赖性的细胞活动中发挥重要作用。本项目拟在此基础上，探讨EB1与CYLD相互作用的分子机制，分析细胞周期进程及细胞迁移信号刺激对二者相互作用的调控；研究EB1与CYLD的相互作用对微管动态性及EB1微管正端跟踪活性的影响；并进一步研究EB1与CYLD的相互作用如何调节微管依赖性的重要细胞活动，包括细胞分裂过程中纺锤体的组装、定位和定向及染色体的捕获、中板集合和分离，以及细胞迁移过程中细胞的极化、细胞前缘稳定微管的形成等。这些研究有助于深入了解微管结合蛋白对微管动态性的调节，也有助于理解微管在细胞分裂、细胞迁移等重要细胞活动中的作用。

Our recent studies have identified an interaction between the microtubule plus end tracking protein EB1 and the novel microtubule-binding protein CYLD and indicated that their interaction might play a role in regulating microtubule dynamics and microtubule-dependent cellular events. In this project, we will investigate the molecular mechanism underlying the EB1-CYLD interaction and analyze how this interaction is regulated by cell cycle progression and cell migration stimuli. We will also study the effects of the EB1-CYLD interaction on microtubule dynamics and the microtubule plus end tracking activity of EB1. In addition, we will investigate how the EB1-CYLD interaction modulates microtubule-dependent cellular activities, including spindle assembly, positioning, and orientation and chromosome capture, congression, and segregation during mitotic progression, and cell polarization and leading-edge stable microtubule formation during cell migration. These studies will help a better understanding of how microtubule-binding proteins regulate microtubule dynamics and how microtubules participate in important cellular events such as cell division and migration.