裂果严重的影响番茄外观品质和商品品质。生产上主要是通过调控外界环境条件减少裂果，从遗传上对果皮开裂的机理研究甚少。本项目组在前期研究中，发现潘那利番茄渐渗系IL4-4果实表皮出现类似于网纹甜瓜表皮的网状裂纹，通过组织石蜡切片、扫描电镜对表型观察，发现裂纹在番茄膨大期（28DPA）开始出现，到绿熟期（35DPA）遍布整个果实表面。本研究利用番茄栽培种M82与IL4-4杂交构建遗传分离群体，通过图位克隆的方法分离番茄果皮网状裂纹控制基因并鉴定其功能，通过酵母单/双杂交、ChIP-Seq等筛选目的基因的上下游调控基因及互作蛋白并进行转基因验证，结合转录组学研究和代谢组学研究，最终阐明番茄中果皮网状裂纹形成的机理，开发分子标记，为选育番茄抗裂新品种奠定基础。

Fruit-cracking seriously affects tomato appearance quality and commodity quality. At present, controlling the environmental condition is the most usually practice to avoid fruit cracking, but it is little known on the mechanism of fruit cracking. Our previous results showed that the fruit skin of IL4-4 shows up netted-cracking like muskmelon. We have observed the structures of fruit cracking by paraffin section and scanning electron microscope, the results revealed that the netted-cracking began to appear as early at 28DPA, and gradually developed, finally reached to all the surfaces of fruit until 35DPA. In this study, we try to clone this gene by map-based cloning approach by F2 population derived from crossing M82 with IL4-4 and further functionally characterize this gene by transgenic analysis. On this basis, we will determine the regulators, interacting proteins and down-stream targets by Y1H, Y2H, ChIP-seq and transgenic analysis. Finally, we integrate the transcriptional and metabolic results to explore the molecular mechanism of fruit-netted cracking, and develop the molecular markers for fruit cracking selection as well.