盆底功能障碍是中老年妇女的常见病，严重影响女性的日常生活。应用合成补片的手术是主要治疗方式之一，但易发生侵蚀、感染等并发症。本课题组前期将脂肪间充质干细胞种植到丝素蛋白支架上建立组织工程补片，动物实验表明其生物相容性与力学性能良好。但存在制备周期长、细胞三维分布难以控制等问题。本课题拟应用目前最先进的3D活细胞生物打印技术来优化盆底组织工程补片的设计及制作，主要研究内容如下： （1）根据影像学资料进行盆底组织或器官的三维重建，建立仿生数字模型；（2）以三维重建模型为模板通过3D打印细胞技术将细胞种植到丝素蛋白支架上构建组织工程补片；（3）打印出完整的细胞-支架复合体，并观察细胞的体外增殖、分泌情况及细胞-支架复合体的生物力学特性；（4）应用恒河猴盆底功能障碍模型研究细胞-支架复合体的生物相容性、力学特征探讨在体组织化、免疫反应及降解后的力学性质改变。构建应用于临床的个体化组织工程补片。

Pelvic function disorder is a common disease among elder female, which has a great impact on their well-beings. Surgeries with synthetic mesh has become the principle way to cure PFD, which proves to induce a couple of complications, such as erosion and infecton. In the former pilot study, we have built an tissue-engineered mesh by implant ADSCs to silk fibrin, which shows both great compatibilities and mechanic characteristic , while the experiment costs a long period ,and shows poor control over cell distribution. Therefore, in the present study, we aim to build up the tissue engineered mesh by the way of 3D bioprinting ,including:（1）3D reconstruction of pelvic organs and tissue based on imaging data;（2）use 3D bioprinting technique implanting the ADSCs onto silk fibrin scaffolds according to the reconstruction model, and observe the proliferation and secretion of the cells , the mechanical characteristics of the cell-scaffold complex ；（3）Macaca rhesus is chosen to set up the IVRO animal model , in order to discuss the biocompatibility mechanic changes of the complex in vivo.