基于表观遗传学的金银花化学质量变异的分子机制研究

药材质量的好坏因其"优劣"评价依据的复杂性，一直是中药领域的难点问题。一般来说，药材"优劣"研究往往侧重于药材指标性成分的高低，或活性成分生物合成途径关键酶基因表达的高低，但相对于药材化学质量的环境、种质、发育阶段、野生与栽培等众多影响因素来说，现有评价指标很难客观的诠释药材的化学质量。 本项目拟在已取得研究成果的基础上，把遗传和环境因素结合起来，分析药材功能基因在内外环境下选择性表达遗传信息的分子机制，以金银花为例开展药材化学质量变异的表观遗传学机制研究及优劣评价标记的筛选，包括金银花及红金银花转录因子结构与功能差异分析、金银花主要化学成分相关生物合成途径关键酶基因5'UTR区的DNA甲基化和组蛋白H3K4位点甲基化分析，筛选与化学质量变异相关的表观遗传标记，为药材"优劣"评价标记的选择提供一条新的思路。

It is important problem that evaluating quality of medicinal materials is difficult in the field of traditional Chinese medicine, because of its complexity. In general, quality of medicinal materials often focuses on the content of active compounds and the gene expression of key enzymes in active compound biosynthetic pathway. Environment, germplasm, developmental stage, wild and cultivated, and many other factors will affect the chemical quality of the medicinal materials. And the existing evaluation is hard to interpret the chemical quality. Based on the pervious works, this project analysis the selective expression of functional gene by combination of genetic and environmental factors. A epigenetic research on chemical quality of Flos Lonicerae was conducted in order to select a marker for quality evaluation