基于调控组的道地黄芩活性成分“逆境效应”形成分子机制研究

作为道地药材形成模式假说之一，逆境效应被认为在道地药材形成中发挥了重要的作用，但目前道地药材形成逆境效应的机制并不清楚。本项目组前期以道地黄芩为例，从“气候因子-基因调控-活性成分”关联入手，建立了“GA-MYB-PAL/CHS-黄酮类活性成分”的调控网络，初步阐明道地黄芩品质形成的环境影响机制。在此基础上，本项目进一步提出了以“SbMYB2/PAL-SbMYB8/CHS”为核心的道地黄芩品质逆境选择性调控假说，拟基于已获得的黄芩cDNA文库和基因组序列，分析不同环境条件下，SbMYB家族成员与bZIP、WRKY、ERF等其他转录因子对黄芩活性成分积累的协同选择性调控作用，建立基于多个转录因子调控组的道地黄芩活性成分“逆境效应”形成基因调控网络，为指导黄芩药材生产、采收提供依据，同时也为道地药材形成环境影响机制研究提供新的思路。

As one of the three powers in TCM system evolution, stress effect is considered to play an important role in daodi-herbs, but about its mechanism unclear. In the past, we used radix scutellariae as example and constructed the gene regulated network GA-MYB-PAL/CHS-flavonoids, according to a link of climate factor, gene regulation and the active ingredient. In this project, we further submit a hypothesis based on a link of SbMYB2/PAL and SbMYB8/CHS, and members of SbMYB family and other transcription factors bZIP、WRKY、ERF could be selected regulation in accumulation of flavonoids under different environment. Based on regulation of multiple transcription factors on active ingredient, the project will construct detail gene regulatory network of daodi Radix scutellariae by stress effect, provide the guiding basis for radix scutellariae medicinal materials production and harvest, and provide new ideas for study environmental impact of daodi-herb conformation.